17-DMAG对鼻咽癌细胞株HNE1增殖与凋亡的影响

目的: 探讨17-DMAG对鼻咽癌HNE1细胞增殖与凋亡的影响。方法: MTT比色法测定17-DMAG对HNE1细胞活性的影响；溴化丙啶(PI)染色法测定HNE1细胞凋亡；JC-1 染色法测定17-DMAG对线粒体膜电位的影响；Western blot测定细胞Bcl-2/Bax的表达水平。结果: 17-DMAG可抑制鼻咽癌HNE1细胞的增殖，且该抑制作用随作用时间的延长和剂量增加而增强；PI染色显示17-DMAG具有诱导HNE1细胞凋亡作用（P<0.01）；JC-1染色显示17-DMAG可诱导HNE1细胞线粒体膜电位降低；Western blot 显示17-DMAG作用HNE1细胞24 h，Bcl-2表达显著降低（P<0.01），Bax表达明显增加，尤其在中、高剂量组（P<0.01）。结论: 17-DMAG具有抑制鼻咽癌细胞HNE1细胞增殖的作用，该抑制作用与浓度和时间成正相关。该作用主要通过诱导HNE1细胞凋亡实现，诱导凋亡的机制可能与其下调细胞线粒体膜电位，降低Bcl-2表达，增加Bax水平有关。

17-DMAG inhibits cell growth and induces apoptosis of nasopharynx cancer cell HNE1

AIM: To explore the effect of 17-DMAG on nasopharynx cancer cell proliferation and apoptosis. METHODS: Cell viability of HNE1 was detected by MTT assay. Cell apoptosis of HNE1 was evaluated by flow cytometry with propidium iodide staining. JC-1 staining was used to determine the effect of 17-DMAG on mitochondrial membrane potential of HNE1 cells. The expressions of Bcl-2 and Bax were detected by Western blot. RESULTS:17-DMAG inhibited the cell proliferation of HNE1 cells in a dose and time-dependent manner. PI staining revealed that 17-DMAG significantly induced HNE1 cell apoptosis（P<0.01）. JC-1 staining showed that 17-DMAG reduced the mitochondrial membrane potential of HNE1 cells. Western blot experiment showed that the expression of Bcl-2 decreased significantly (P<0.01). The expression of Bax increased significantly, especially in middle and high dose group after HNE1 cells being treated with 17-DMAG for 24 h (P<0.01). CONCLUSION: 17-DMAG inhibits the growth of HNE1 cells in a dose and time-dependent manner by inducing the apoptosis of HNE1 cells. The mechanism is related with reducing the mitochondrial membrane potential and the expression of Bcl-2, and increases the level of Bax.