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| 酒醅中产纤溶酶细菌的分离及发酵产酶研究 | |
| 引用本文： | 邓永平,杨 敏,郭建华,李 琰,刘晓兰,艾瑞波.酒醅中产纤溶酶细菌的分离及发酵产酶研究[J].中国酿造,2017,36(5):67. |
| 作者姓名： | 邓永平 杨 敏 郭建华 李 琰 刘晓兰 艾瑞波 |
| 作者单位： | 1.齐齐哈尔大学食品学院，黑龙江齐齐哈尔161006； 2.黑龙江省普通高校农产品深加工重点实验室，黑龙江齐齐哈尔161006； 3.齐齐哈尔大学理学院，黑龙江齐齐哈尔161006 |
| 基金项目： | 齐齐哈尔市科学技术计划项目（SFGG-201578） |
| 摘 要： | 该研究从酒醅中筛选高产纤溶酶的细菌。经过初筛和复筛得到1株高产纤溶酶的菌株，命名为DL-1。通过菌株形态学和生理生化试验初步鉴定菌株DL-1为芽孢杆菌（Bacillus sp.）。菌株DL-1产纤溶酶发酵试验结果表明，最适菌龄为4 h，最适碳源为牛肉膏，最适氮源为豆饼粉。在此条件下，所产的纤溶酶在血纤维蛋白平板上形成的溶圈面积可达379.79 mm2。 |
| 关 键 词： | 酒醅 纤溶酶 细菌 分离 鉴定 |
Isolation of fibrinolytic enzyme-producing bacterium from the fermented grains and its enzyme production |
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| DENG Yongping,YANG Min,GUO Jianhua,LI Yan,LIU Xiaolan,AI Ruibo.Isolation of fibrinolytic enzyme-producing bacterium from the fermented grains and its enzyme production[J].China Brewing,2017,36(5):67. | |
| Authors: | DENG Yongping YANG Min GUO Jianhua LI Yan LIU Xiaolan AI Ruibo |
| Affiliation: | 1.College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China; 2.Key Laboratory of Processing Agricultural Products of Heilongjiang Province, Qiqihar 161006, China; 3.College of Science, Qiqihar University, Qiqihar 161006, China |
| Abstract: | The high yield fibrinolytic enzyme-producing bacteria were isolated from fermented grains for alcoholic drink. A high yield fibrinolytic enzyme-producing strain was obtained by preliminary screening and secondary screening and named as DL-1. The strain DL-1 was preliminarily identified as Bacillus sp. by morphological and physiological and biochemical experiments. The results of fibrinolytic enzyme-producing fermentation tests of strain DL-1 indicated that the optimum cell age was 4 h, the optimum carbon source was beef extract, and the optimum nitrogen source was soybean cake powder. Under the condition, the soluble ring area of fibrinolytic enzyme in the blood fibrin plate reached to 379.79 mm2. |
| Keywords: | fermented grains fibrinolytic enzyme bacteria isolation identification |
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