加味青娥丸对2型糖尿病大鼠心肌组织的保护作用

目的:观察加味青娥丸(Modified Qing'e Pill)对糖尿病(diabetes mellitus,DM)模型大鼠糖脂代谢及其心肌组织功能的影响,并初步探讨其可能机制。方法:将健康SD大鼠（共72只）随机分为6组（n=12）：正常组（正常喂养、未建模）、模型组、加味青娥丸低、中、高三个剂量组（剂量分别为：2.7、5.4、10.8 g/kg）、二甲双胍组（0.15 g/kg）,采取“高脂喂养”+“腹腔注射链脲菌素（STZ）”的方法进行建模。给药方式均为灌胃,频率均为每天1次,时长均为8周,其中：加味青娥丸低、中、高三个剂量组和二甲双胍组给予对应药物灌胃,而正常组和模型组则给予等量的生理盐水灌胃。由专人分别在固定时间点（给药前、第4周、第8周）观察各组大鼠精神状态、称量各组大鼠体质量、测定各组大鼠空腹血糖（FBG）、餐后2 h血糖（2PBG）;给药周期结束后2 h,对血清中相关指标（糖化血红蛋白、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、甘油三酯、总胆固醇、超敏 c反应蛋白、TNF-α）进行测定;给药周期结束后2 h,对心肌组织炎症、氧化应激相关指标（MDA、SOD、GSH）进行测定。结果: 给药组相比于模型组而言,大鼠精神状态、体质量、血糖及脂质水平均有所改善。同时,给药组能够降低血清中hs-CRP、TNF-α的水平,提高心肌组织中SOD、GSH的含量,降低心肌组织中MDA的含量。结论:加味青娥丸可以改善2型糖尿病SD大鼠的糖脂代谢,通过抗炎、抗氧化改善心肌损伤,保护心肌组织。

Protective effects of Modified Qing'e Pill on myocardial tissue in type 2 diabetic rats

AIM: To observe the effects of Modified Qing'e Pill on glucolipid metabolism and myocardium function in type 2 diabetic rats. METHODS: Seventy-two healthy similar weighted SD rats were randomly divided into six groups: the normal group, the model group, low dose group (2.7 g/kg), middle dose group (5.4 g/kg), high dose group (10.8 g/kg) and Metformin group (0.15 g/kg). Model of Diabetes mellitus was set by "high fat diet" + "intraperitoneal injection of streptozotocin (STZ)". All groups received intragastric administration once per day for eight weeks. Groups of Modified Qing'e Pill and Metformin group were given the corresponding dose of drugs, while the normal and model groups were given the same volume of saline. At the fixed time points ( pre-administration, the 4th week, the 8th week) , the mental state, the weight of the rats, the FBG and 2PBG of rats were observed and detected; 2 h after the end of administration, relevant indicators (HbA1c,LDL,HDL,TG,TC,hs-CRP,TNF-alfa) in serum and the relevant indicators (MDA, SOD, GSH) in myocardial tissue were determined. RESULTS:Compared with the model group, mental state, weight, blood glucose and lipid levels were all improved of rats in the administration group. Also, Modified Qing'e Pill could reduce the levels of hs-CRP and TNF-alfa in serum, increase the content of SOD and GSH in myocardium and decrease the content of MDA in myocardium. CONCLUSION: Modified Qing'e Pill can improve the glucose and lipid metabolism in type 2 diabetic rats, and protect myocardial tissue from injury through anti-inflammation and antioxidation.