黄芪总苷对糖尿病小鼠肾脏保护作用的研究

目的:研究黄芪总苷（AST）对糖尿病小鼠肾脏的保护作用及其可能的作用机制。方法:链脲佐菌素（STZ）诱导建立糖尿病小鼠模型。随机分为模型组、4-羟基-2,2,6,6-四甲基哌啶组，AST 低、中、高三个剂量组。五组动物分别在给药后4周、6周，检测空腹血糖浓度（FBG）及糖化血清蛋白含量（GSP），称量体质量，计算肾脏指数，观察肾脏病理改变，TUNEL法检测肾小球细胞凋亡，RT-PCR法检测肾脏转化生长因子-β1（TGF-β1）、IV型胶原蛋白（Col IV） mRNA表达情况。 结果:AST 能显著降低STZ诱导的糖尿病小鼠FBG、GSP，且呈现时间、剂量依赖性（P<0.01）。给药后6周，各用药组小鼠体质量上升，肾脏指数下降，肾小球 PAS阳性分值下降，肾小球细胞凋亡率降低（P<0.01）。与模型组比较，AST中剂量组小鼠肾脏Col Ⅳ、TGF-β1 mRNA 表达水平明显下降（P<0.05）。结论:AST对糖尿病小鼠有肾脏保护作用，其机制可能与其促进肾脏Col Ⅳ、TGF-β1 mRNA表达有关。

Protective effect of astragalosides on kidney damage in diabetic mice

AIM: To study the effect of astragalosides (AST) on kidney damage and the mechanism in diabetic mice. METHODS: The diabetic mice model was established by intraperitoneal injection with STZ and the model mice were randomly divided into model group, tempol group, AST-L group, AST-M group, and AST-H group. After 4 weeks and 6 weeks of administration, concentration of fasting blood glucose (FBG) and glycosylated serum protein (GSP) were recorded; weight and kidney indexes were calculated; renal pathological changes were observed; glomerular apoptosis and renal TGF-beta 1, Col Ⅳ mRNA expressions were detected by TUNEL and RT-PCR method, respectively. RESULTS: AST can significantly reduce the levels of serum FBG and GSP of diabetic mice induced by STZ in time and dose dependent way (P<0.01). After 6 weeks of administration, the body weight increased, while the kidney index, glomerulus defects and cell apoptosis decreased in all treatment group(P<0.01). Compared with model group, the high expression of Col Ⅳ mRNA and TGF-β1 mRNA were significantly decreased in kidney of AST 60 mg/kg dose group (P<0.05). CONCLUSION: There are some protective effects of AST on kidney of experimental diabetic mice, and its mechanism may be related to promote kidney Col Ⅳ, TGF-beta 1mRNA expression.