| 凝胶过滤层析分离纤维低聚糖 |
| |
| 引用本文： | 彭红,林鹿,邓海波,孙勇,孙润仓. 凝胶过滤层析分离纤维低聚糖[J]. 食品与发酵工业, 2007, 33(2): 137-140 |
| |
| 作者姓名： | 彭红 林鹿 邓海波 孙勇 孙润仓 |
| |
| 作者单位： | 华南理工大学制浆造纸工程国家重点实验室,广东广州,510640 |
| |
| 基金项目： | 高校科技创新工程项目;教育部长江学者和创新团队发展计划 |
| |
| 摘 要： | 纤维低聚糖具有重要的生理功能,是一种功能性食品基料。低聚糖的分离方法有很多种,柱层析是分离低聚糖的重要方法之一。利用聚丙烯酰胺凝胶柱(Bio-Gel P-2)层析能将水溶性纤维低聚糖混合物很好的分离开来,二次分离可得到白色的纤维二糖~五糖的单一组分的。在水-乙醇溶液中,纤维五糖和四糖析出的白色固体粘附在瓶壁上,析出的纤维三糖和纤维二糖成白色粉末状。
|
| 关 键 词： | 纤维低聚糖 聚丙烯酰胺凝胶 聚合度 分离 |
| 修稿时间： | 2006-11-14 |
Isolation of Cellooligosaccharides by Gel Filtration |
| |
| Peng Hong,Lin Lu,Deng Haibo,Sun Yong,Sun Runcang. Isolation of Cellooligosaccharides by Gel Filtration[J]. Food and Fermentation Industries, 2007, 33(2): 137-140 |
| |
| Authors: | Peng Hong Lin Lu Deng Haibo Sun Yong Sun Runcang |
| |
| Abstract: | Cellooligosaccharides is a kind of functional food with important biological activities.Many methods for the separation were applied,one of which is column chromatography.The results showed that cellooligosaccharides could be isolated on a polyacrylamide gel(Bio-Gel P-2) column.The pure oligosaccharides such as cellobiose,cellotriose,cellotetraose and cellopentaose were obtained after another time separation.White solid celloteraose and cellopentaose were precipitated in water-ethanol solvent,adhering to the bottom of the bottle.Pure cellotriose and cellobiose were precipitated as white powder. |
| |
| Keywords: | cellooligosaccharides polyacrylamide gel degree of polymerization separation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
