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| 4个引进甜菜品种DNA指纹图谱的建立 | |
| 引用本文： | 刘巧红,吴玉梅,罗成飞,刘乃新,杨林,王皙玮,袁肖寒.4个引进甜菜品种DNA指纹图谱的建立[J].中国甜菜糖业,2011(2):5-7. |
| 作者姓名： | 刘巧红 吴玉梅 罗成飞 刘乃新 杨林 王皙玮 袁肖寒 |
| 作者单位： | 1. 哈尔滨工业大学食品科学与工程学院,黑龙江,哈尔滨,150086 2. 黑龙江大学农作物研究院/中国农业科学院甜菜研究所/农业部甜菜品质监督检验测试中心,黑龙江,哈尔滨,150080 3. 东北农业大学生命科学与生物技术研究中心,黑龙江,哈尔滨,150030 |
| 基金项目： | 国家甜菜产业技术体系项目，国家及黑龙江省自然科学基金，黑龙江省教育厅项目，国家科技项目，哈尔滨市科技创新人才研究专项资金项目 |
| 摘 要： | 利用ISSR标记技术，对4个引进的甜菜品种建立DNA指纹图谱。结果显示，用筛选出的1条引物的PCR扩增指纹图谱即可将这4个品种区分，该引物共扩增出12个清晰条带，其中6个为共有带，多态性为50％，4个品种分别有自己的特征带。研究结果表明，利用ISSR标记指纹能够从DNA水平上明确区分甜菜不同品种，为甜菜种质鉴定提供了一种有效方法。 |
| 关 键 词： | 甜菜 ISSR DNA指纹 |
Establishing of DNA fingerprint for 4 introduced sugar beet varieties |
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| LIU Qiao-hong,WU Yu-mei,LUO Cheng-fei,LIU Nai-xin,YANG Lin,WANG Xi-wei,YUAN Xiao-han.Establishing of DNA fingerprint for 4 introduced sugar beet varieties[J].China Beet & Sugar,2011(2):5-7. | |
| Authors: | LIU Qiao-hong WU Yu-mei LUO Cheng-fei LIU Nai-xin YANG Lin WANG Xi-wei YUAN Xiao-han |
| Affiliation: | LIU Qiao - hong , WU Yu - mei, LUO Cheng - fei, LIU Nai - xin, YANG Lin, WANG Xi - wei, YUAN Xiao -han( 1. School of Food Science and Engineering, Harbin institute of Technology, Harbin 150086, China ; 2. Institute for Crop Research, HeilongjiangUniversity, Sugarbeet Research Institute of China Acadamic of Agriculture Sciences/Center for the Control of Sugarbeet Quality, Ministry of Agriculture, P. R. , Harbin 150080, China ;3. Life Sciences and Biotechnique Research Center, Northeast Agricultural University, Harbin 150030,China) |
| Abstract: | DNA fingerprints of 4 introduced sugar beet varieties were constructed using ISSR mark technology. The result shows, the fingerprint formed by PCR with only one selected primer can distinguish four sugar beet varieties ; 12 bands were scored, the polymorphism was 50% ; 4 varieties all have its own specific band. The research suggest that DNA fingerprint of ISSR can distinguish different kind of sugar beet varieties in DNA level and it provides a effective method for sugar beet germplasm identification. |
| Keywords: | sugar beet ISSR DNA fingerprint |
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