| ~(131)I标记新型小分子肽VP2 |
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| 引用本文： | 马欢,唐禹,杨远友,刘宁,廖家莉,杨吉军.~(131)I标记新型小分子肽VP2[J].同位素,2016,29(1):1-5. |
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| 作者姓名： | 马欢 唐禹 杨远友 刘宁 廖家莉 杨吉军 |
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| 作者单位： | 四川大学原子核科学技术研究所辐射物理及技术教育部重点实验室,四川成都,610064 |
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| 基金项目： | 国家自然科学基金(21371124 |
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| 摘 要： | 本文通过双功能偶联剂5-(三正丁基锡)-3-吡啶甲酸-N-琥珀酰亚胺酯(SPC)将131I标记到小分子融合多肽VP2上,研究了131I标记多肽VP2的体内外稳定性及在正常小鼠体内的代谢与分布。结果表明,该标记药物室温下放置48h后放化纯度仍可达97%,其在小鼠体内可通过胃肠道快速代谢,在甲状腺的摄取较低。用间接标记法得到的131I-SPC-VP2在体内外有良好的稳定性。
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| 关 键 词： | 131I VP2 标记药物 小分子肽 生物分布 |
Preliminary Study of Radioiodination of VP2 |
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| MA Huan,TANG Yu,YANG Yuan-you,LIU Ning,LIAO Jia-li,YANG Ji-jun.Preliminary Study of Radioiodination of VP2[J].Isotopes,2016,29(1):1-5. |
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| Authors: | MA Huan TANG Yu YANG Yuan-you LIU Ning LIAO Jia-li YANG Ji-jun |
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| Affiliation: | Key Laboratory of Radiation Physics and Technology, Ministry of Education,Institute of NuclearScience and Technology, Sichuan University, Chengdu 610064, China |
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| Abstract: | The VP2 peptide specifically binding to vasoactive intestinal polypeptide receptor 1(VPAC1) receptor was radioiodinated with131I by bi-functional linker N -succinimidyl 5-(tributylstannyl)-3-pyridinecarboxylate (SPC), and in vitro and in vivo stability of the labeled peptide were evaluated. Mice were injected intravenously with either 1.2×106 Bq 131I-SPC-VP2 or Na 131I. Radiochemical purity and biodistribution were studied at various time points. The result showed that purified 131I-SPC-VP2 exhibited radiochemical purity of 97% at room temperature up to 48 h. The tissue uptake of131I-SPC-VP2 was low after 6 h injection, especially in thyroid. The result indicated the labeled compound had fast clearance and good stability in vivo. |
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| Keywords: | 131I VP2 labeled compound peptide biodistribution |
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