NOX 4对高糖代谢乳腺癌细胞侵袭转移能力的作用

利用2-氟［¹⁸F-2］脱氧葡萄糖（［¹⁸F］fluorodeoxyglucose,¹⁸F-FDG）乳腺癌细胞摄取实验筛选高糖酵解率的乳腺癌细胞；采用基因沉默技术，降低细胞烟酰胺腺嘌呤二核苷酸磷酸氧化酶4 (NOX 4)表达；采用免疫印迹方法检测三组细胞（空白对照组、非特异性siRNA转染组和特异性NOX 4-siRNA转染组）的上皮间质转化（epithelial mesenchymal transition, EMT）相关分子HIF-1α和TGF-β表达水平；通过细胞侵袭实验分析三组细胞的侵袭能力差异。结果显示,高侵袭力的MDA-MB-231较低侵袭力的MCF-7乳腺癌细胞系具有高糖酵解率（t=10.52,P<0.05）；抑制MDA-MB-231细胞的NOX 4表达，降低了细胞EMT相关因子HIF-1α、TGF-β表达（P均<0.01）与细胞侵袭能力（t=-8.0, P<0.01）。本研究初步证实NOX 4失活能够降低高糖代谢乳腺癌细胞的侵袭转移能力。

Effect of NOX 4 on the Invasion and Metastasis of Breast Cancer Cells with High Glucose Metabolism

The breast cancer cells with high glucose metabolism were screened by 18 F-FDG cell uptake experiment .The gene silencing technique was used to reduce NOX 4 expression in cells .The experiment was divided into three groups :control group ,the non-specific transfection group and specific transfection group .Western blotting was used to detect differences in the expression of HIF-1α and TGF-βin three groups .The invasion ability of the three groups by transwell cell invasion assay was analyzed .The results showed that MDA-MB-231 breast cancer cells had a higher rate of glycolysis than MCF-7 tumor cells(t=10.52 ,P<0.05) .The expression of HIF-1αand TGF-βand the invasiveness of cells were reduced by inhibiting the level of NOX 4 .This study showed that inhibition of NOX 4 expression was able to inhibit the invasion of breast cancer cell with high glucose metabolism .