鼠脑肿瘤血卟啉光敏诊治时间的探索

目的:寻求鼠脑肿瘤注血卟啉后光敏诊治最佳时间。方法:在注HPD后不同时间对正常脑组织和脑肉瘤组织用氙离子激光激发荧光,采用OMA系统分析观察两组的荧光变化。结果:显示注药3小时后小鼠正常脑组织血卟啉荧光峰明显下降而脑肉瘤组织仍保持较高血卟啉浓度。结论:注药后4~5小时是最佳的鼠脑肿瘤光敏诊断及光动力学治疗的时间。

Research of had best time of HPD-PDT and photosensitive diagnose of S180 sarcoma in brain

Objective: Search the metabolic comparision of HPD in normal brain tissue and S180 sarcoma in brain. Methods:Xe laser photoradiation of normal brain tissue and S180 sarcoma in brain to excite fluorescence was performed at different time after the injection of hematoporphyrin derivative (HpD) in normal rats and rats with S180 sarcoma of Brain. We analysed HPD fluorescence lifetime imaging (wavelength: 630 nm and 690 nm) of two rat team with OMA.Result:We concluded the peak intensity of HPD fluorescence fell obviously in normal brain three hours later after injection, while it still retained high HPD concentration in sarcoma of brain. Conclusion:first-rank time the 4~5 hours after injection of HPD is first-rank time 0f PDT and photosensitive diagnose in sarcoma of brain of rat.