Identifizierung von Glucosinolaten in Rettich (Raphanus sativus L.) und Kreuzungen aus R. sativus L. x Brassica oleracea L. (Raphanobrassica) mittels LC-MS

Identification of glucosinolates in radish (Raphanus sativus L.) and cross-breeds of R. sativus L. x Brassica oleracea L. (Raphanobrassica) by LC-MS. The glucosinolate profiles in Raphanus sativus and new intergenetic as well as interspecific hybrids between Raphanus sativus and Brassica oleracea have been investigated by HPLC-UV and LC-MS. Tentative identification based on UV-spectra and retention times were confirmed by the indivdual LC-MS measurements. In this context three glucosinolates (glucoraphenin, glucoerysolin and 4-methylthiobutenyl glucosinolate), which are known for their nutritional and resistance value in the brassicaceae plants, but are not available as reference standards could be characterized by MS detection for the first time. The presented LC-MS analyses, performed in the API-ES positive mode, proved to be a very sensitive and selective method for the identification of unknown glucosinolates in plant material. Possible applications for breeding research as well as for quality control purposes are discussed.