| RP-HPLC测定人参茎叶总皂苷及不同部位浸膏中Rb_3的质量分数 |
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| 引用本文： | 马畅,申贵隽,李珂珂,陈丽荣,弓晓杰.RP-HPLC测定人参茎叶总皂苷及不同部位浸膏中Rb_3的质量分数[J].大连工业大学学报,2013(3):180-182. |
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| 作者姓名： | 马畅 申贵隽 李珂珂 陈丽荣 弓晓杰 |
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| 作者单位： | 大连大学环境与化学工程学院;大连大学医学院 |
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| 基金项目： | 国家自然科学基金资助项目(81172949);辽宁省教育厅科研项目(LT2010003;L2012439);大连市科技计划项目(2010E12SF061;2011E12SF038) |
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| 摘 要： | 采用反相高效液相色谱法(RP-HPLC)测定人参不同部位浸膏及人参茎叶总皂苷中人参皂苷Rb3的质量分数。色谱柱,Nava-Pak-C18(3.9mm×150mm,4μm);流动相,V(甲醇)∶V(水)∶V(磷酸)=65∶35∶1;检测波长,203nm;体积流量,1.0mL/min;采用外标法测定人参皂苷Rb3质量分数。结果表明,人参皂苷Rb3在0.8～20.0μg呈良好的线性关系(R=0.999 9),平均加样回收率为100.3%。
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| 关 键 词： | 人参茎叶总皂苷 人参皂苷Rb3 反相高效液相色谱 |
Determination of total ginsenosides from the stems and leaves and ginsenoside Rb_3 in different extracts of Panax ginseng by RP-HPLC |
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| MA Chang,SHEN Guijun,LI Keke,CHEN Lirong,GONG Xiaojie.Determination of total ginsenosides from the stems and leaves and ginsenoside Rb_3 in different extracts of Panax ginseng by RP-HPLC[J].Journal of Dalian Dalian Polytechnic University,2013(3):180-182. |
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| Authors: | MA Chang SHEN Guijun LI Keke CHEN Lirong GONG Xiaojie |
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| Affiliation: | 1.College of Environment and Chemical Engineering,Dalian University,Dalian 116622,China; 2.Medical College,Dalian University,Dalian 116622,China) |
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| Abstract: | RP-HPLC method was developed to determine ginsenoside Rb3 in different extracts of Panax ginseng and total ginsenosides from the stems and leaves of Panax ginseng on Nava-Pak ODS column(3.9 mm×150 mm,4 μm).The mobile phase was V(methanol)∶V(water)∶V(phosphoric acid)=65∶35∶1,the detection wavelength was set at 203 nm,the flow rate was 1.0 mL/min.The content of ginsenoside Rb3 was determined with external standard method.The ginsenoside Rb3 had a good linearity from 0.8 to 20.0 μg(R=0.999 9),and the average recovery was 100.3%. |
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| Keywords: | total ginsenosides of Panax ginseng ginsenoside Rb3 RP-HPLC |
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