| Abstract: | Listeria monocytogenes(L.monocytogenes)is one of the top five dangerous foodborne pathogens which widely exists in most raw food and has approximately 30％mortality rate in high-risk groups.Food safety caused by foodborne pathogens is still a major problem faced by humans in all world.The conven-tional analytical methods currently used involve complex bacteriological tests and usually take several days for incubation and analysis.Thus,in order to prevent the spread of disease,the development of a detection method with high speed,high accuracy and sensitivity is urgent and necessary.Herein,we developed an approach for the identification and magnetic capture of L.monocytogenes by using core@shell Fe3O4@silica nanoparticles terminated with hydroxyl or amine groups.Our results show that both amine-and hydroxyl-terminated Fe3O4@silica core@shell nanoparticles functionalized with spe-cific antibodies,present 95.2％±6.2％and 98.6％±0.3％capture efficacies,respectively.However,without conjugating the specific antibodies,the hydroxyl-terminated Fe3O4@silica nanoparticles exhibit 17.6％±1.6％efficacy,while the amine-terminated one remains 93.2％±9.2％capture efficiency ascribed to the high affinity.This study quantitatively uncovers the specific and non-specific recognitions relevant to the molecular-scale physiochemical interactions between the microorganisms and the functionalized particles,and the results from this work can be generalized and extended to other bacterial species by changing antibodies,also have important implications in developing advanced analytic methods. |
