| 采用圆二色谱法研究冬夏鲢鱼鳞胶原蛋白的稳定性 |
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| 引用本文： | 段蕊,叶超,邢芳芳,闻海波,今野久仁彦.采用圆二色谱法研究冬夏鲢鱼鳞胶原蛋白的稳定性[J].食品与发酵工业,2010(1). |
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| 作者姓名： | 段蕊 叶超 邢芳芳 闻海波 今野久仁彦 |
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| 作者单位： | 淮海工学院海洋学院;江苏省海洋资源开发研究院;北海道大学水产科学研究院; |
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| 基金项目： | 江苏省连云港市科技局资助项目(项目编号#CG0803-3); 江苏省海洋资源开发研究院资助项目(项目编号#JSIMR09D04); 日本学术振兴会(JSPS)资助; 连云港市科技局的资助 |
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| 摘 要： | 采用圆二色谱法,研究比较了冬夏季鲢鱼鳞胶原蛋白的热稳定性。结果表明,对胶原蛋白溶液于30℃,33℃加热时,冬季鱼鳞胶原蛋白发生解螺旋现象,而夏季鱼鳞胶原仍保持完整的三螺旋结构;变性温度曲线显示,夏季样品的变性温度为36℃,而冬季样品出现2个峰值,分别为30℃和34℃。结果表明,夏季鲢鱼鳞胶原蛋白的热稳定性高于冬季胶原蛋白,这种不同可能与冬夏季鲢鱼生长环境温度的差异有关。
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| 关 键 词： | 鲢鱼鳞 胶原蛋白 圆二色谱 稳定性 |
Comparison on Stability of Scale Collagens from Silver Carp (Hypophthalmichthys molitrix) by Circular Dichroism |
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| Duan Rui,Ye Chao,Xing Fangfang,Wen Haibo,Konno Kunihiko.Comparison on Stability of Scale Collagens from Silver Carp (Hypophthalmichthys molitrix) by Circular Dichroism[J].Food and Fermentation Industries,2010(1). |
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| Authors: | Duan Rui Ye Chao Xing Fangfang Wen Haibo Konno Kunihiko |
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| Affiliation: | Duan Rui1,2,Ye Chao1,Xing Fangfang1,Wen Haibo1,Konno Kunihiko31(School of Marine Science , Technology,Huaihai Institute of Technology,Lianyungang 222005,China)2(Jiangsu Marine Resource Development Research Institute. Lian Yungang 222005,China)3(Faculty of Fisheries Sciences,Hokkaido University,Hakodate,Hokkaido 041-8611,Japan) |
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| Abstract: | Scales of silver carp(Hypophthalmichthys molitrix)were collected in winter and summer.Acid-soluble collagens(ASC)were extracted from the scales collected from the two seasons,respectively.Thermal stability of the collagens was investigated by changes in circular dichroism(CD)spectrum upon heating.The CD measurement results showed different thermostability of winter and summer samples heated at 30,33 and 35℃.The melting temperature of scale ASC from summer fish was 36 ℃,while scale ASC from winter fish showed the existence of biphasic thermal transitions with the melting temperature of 30 and 34 ℃.It was concluded scale ASC from the summer group was more stable than its equivalent from the winter season.The difference might be the consequence of the need to adapt to the habitating environment. |
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| Keywords: | scale of silver carp collagen stability circular dichroism |
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