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| 植物乳杆菌素抑菌机理的研究 | |
| 引用本文： | 程建军,李想,郭明若.植物乳杆菌素抑菌机理的研究[J].中国乳品工业,2011,39(5). |
| 作者姓名： | 程建军 李想 郭明若 |
| 作者单位： | 1. 东北农业大学,哈尔滨,150030 2. 双城雀巢有限公司,哈尔滨,150100 3. 佛蒙特大学,美国,伯灵顿,05403 |
| 摘 要： | 从植物乳杆菌B-28的代谢产物中分离纯化植物乳杆菌素,以蜡样芽胞杆菌为指示菌,通过测定指示菌细胞结构和细胞中APT、离子量的变化,分析探讨植物乳杆菌素的抑菌机理。研究结果表明:当植物乳杆菌素作用于蜡样芽胞杆菌时,胞内ATP呈现迅速下降趋势;并迅速释放出K+和Na+,而且植物乳杆菌素的质量分数与K+和Na+释放量基本呈现线性正相关。随着植物乳杆菌素添加量的增加,蜡样芽胞杆菌细胞裂解率在逐渐提高。而且电镜观察发现:植物乳杆菌素作用的蜡样芽胞杆菌的细胞壁边缘模糊,菌体细胞变形,部分细胞壁缺失,可看到胞内物质的损失。 |
| 关 键 词： | 植物乳杆菌素 抑菌 机理 |
Antibacterial mechanism of plantaricin by Lactobacillus plantarum B-28 |
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| CHENG Jian-jun,LI Xian,GUO Ming-ruo.Antibacterial mechanism of plantaricin by Lactobacillus plantarum B-28[J].China Dairy Industry,2011,39(5). | |
| Authors: | CHENG Jian-jun LI Xian GUO Ming-ruo |
| Affiliation: | CHENG Jian-jun1,LI Xiang2,GUO Ming-ruo3(1.Northeast Agricultural University,Harbin 150030,China,2.Nestle Shuangcheng Limited Company,Harbin 150100,3.University of Vermont,Burlington,VT 05403,USA) |
| Abstract: | The plantarcin was screened by Lactobacillus plantarum B-28.The Bacillus cereus was the indicator bacteria.The antibacterial mechanism of the plantarcin was investigated through the changes of ATP level,ionic content and structure of cell.The results showed: As plantarcin which was screened by Lactobacillus plantarum B-28 was added,intracellular ATP levels in energized cells of the Bacillus cereus dropped quickly and then tended to be stable,however,extracellular ATP level increased during incubation in the... |
| Keywords: | plantaricin antibacterial mechanism |
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