| 乳品中肠球菌LAMP快速检测方法的建立及应用 |
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| 引用本文： | 姜侃,张东雷,金燕飞,陈小珍.乳品中肠球菌LAMP快速检测方法的建立及应用[J].中国乳品工业,2011,39(5). |
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| 作者姓名： | 姜侃 张东雷 金燕飞 陈小珍 |
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| 作者单位： | 浙江省质量技术监督检测研究院,杭州,310013 |
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| 基金项目： | 浙江省科技厅优先主题重点社会发展项目(No.2009C13013); 浙江省质量技术监督系统科研项目(No.20090209) |
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| 摘 要： | 应用环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP),针对肠球菌16sRNA中高度保守的序列分析设计特异性引物,建立乳品中肠球菌特异性快速检测方法。结果表明,所设计的引物具有良好特异性,10株肠球菌均能扩增出特异性片段,而14株非肠球菌均未扩增出相应片段,无假阴性或假阳性情况出现。同时,该方法可在14 h内完成检测,且阳性LAMP反应所需目标菌DNA浓度仅为10 fg/μL。应用本方法对164份婴幼儿配方乳粉的检测结果显示,共在37个批次中检出肠球菌,检出率为22.6%,污染量在0.36~110 g-1(最近似值)间。该方法为肠球菌的快速检测提供了一种重要的技术手段。
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| 关 键 词： | 肠球菌 LAMP 快速检测 乳品 |
Establishment of LAMP rapid detection method for Enterococcus in dairy products |
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| JIANG Kan,ZHANG Dong-lei,JIN Yan-fei,CHEN Xiao-zhen.Establishment of LAMP rapid detection method for Enterococcus in dairy products[J].China Dairy Industry,2011,39(5). |
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| Authors: | JIANG Kan ZHANG Dong-lei JIN Yan-fei CHEN Xiao-zhen |
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| Affiliation: | JIANG Kan,ZHANG Dong-lei,JIN Yan-fei,CHEN Xiao-zhen(Zhejiang Test Academy of Quality and Technical Supervision,Hangzhou 310013,China) |
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| Abstract: | LAMP(Loop-mediated isothermal amplification,LAMP) rapid detection method for Enterococcus in dairy products was established,with the LAMP primers designed based on the 16sRNA sequence.The results of the study indicated that the method had perfect specificity,as 10 strains of Enterococcus were able to amplify specific fragments,while 14 strains of non-Enterococcus were not,and no false positive or false negative results occurred.The test could be done within 14 hours,and the concentration of target bacteria ... |
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| Keywords: | Enterococcus LAMP rapid detection dairy |
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