| miR-126对小鼠乳腺上皮细胞增殖及泌乳功能的影响 |
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| 引用本文： | 崔巍,王春梅,李庆章,冯丽,丁巍. miR-126对小鼠乳腺上皮细胞增殖及泌乳功能的影响[J]. 中国乳品工业, 2011, 39(3) |
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| 作者姓名： | 崔巍 王春梅 李庆章 冯丽 丁巍 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金(No.31072103) |
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| 摘 要： | 应用脂质体转染技术,改变miR-126在小鼠乳腺上皮细胞中的表达量,采用qRT-PCR、HPLC、Western印迹等技术检测miR-126对小鼠乳腺上皮细胞的影响。结果显示,miR-126沉寂后,细胞增殖能力提高,β-酪蛋白表达增加,乳糖质量浓度增加,孕酮受体(PGR)表达增强。研究表明,miR-126可能通过抑制靶蛋白PGR表达,进而抑制乳腺上皮细胞增殖和泌乳量变化。
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| 关 键 词： | 微小RNA miR-126 孕酮受体(PGR) 基因沉寂 |
Impact of miR-126 on proliferation and lactation of Mouse mammary epithelial cells |
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| CUI Wei,WANG Chun-mei,LI Qing-zhang,FENG Li,DING Wei. Impact of miR-126 on proliferation and lactation of Mouse mammary epithelial cells[J]. China Dairy Industry, 2011, 39(3) |
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| Authors: | CUI Wei WANG Chun-mei LI Qing-zhang FENG Li DING Wei |
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| Affiliation: | CUI Wei,WANG Chun-mei,LI Qing-zhang,FENG Li,DING Wei(Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | In this study,liposome transfection techniqueswere used to change the expression of miR-126 in mouse mammary epithelial cells.The effect of miR-126 on mouse mammary epithelial cells was analyzed by qRT-PCR,HPLC,Western blotting.The results showed that,after miR-126 silence cell proliferation ability enhanced,beta casein expression increased,lactose content increased,progesterone receptor(PGR) expression enhanced.The results suggest that miR-126 probably inhibit mammary epithelial cells and lactation by supp... |
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| Keywords: | microRNA miR-126 progestrone receptor gene silencing |
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