| 奶牛乳腺中瘦素及其受体的表达与定位 |
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| 引用本文： | 郭洪波,林叶,李庆章,边艳杰,肖阳. 奶牛乳腺中瘦素及其受体的表达与定位[J]. 中国乳品工业, 2011, 39(6) |
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| 作者姓名： | 郭洪波 林叶 李庆章 边艳杰 肖阳 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金资助项目(30671538); 东北农业大学创新团队项目(CXT005-1-1/CXT005-1-2) |
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| 摘 要： | 为探讨瘦素在奶牛乳腺发育、泌乳及退化各时期的表达变化规律及在乳腺组织中的具体位置,采用免疫印迹(western blot-ting)技术及激光共聚焦技术检测奶牛乳腺组织中瘦素及瘦素受体(OB-Rb)的表达变化及其定位。结果表明,瘦素在青春期表达量较高,在泌乳期表达量最低。瘦素受体在青春期和妊娠期表达量较高,泌乳期表达量相对较低,退化期逐渐恢复到妊娠期水平。
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| 关 键 词： | 奶牛 乳腺组织 瘦素 长型瘦素受体 |
Expression and localization of leptin and its receptor in mammary gland of dairy cow |
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| GUO Hong-bo,LIN Ye,LI Qing-zhang,BIAN Yan-jie,XIAO Yang. Expression and localization of leptin and its receptor in mammary gland of dairy cow[J]. China Dairy Industry, 2011, 39(6) |
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| Authors: | GUO Hong-bo LIN Ye LI Qing-zhang BIAN Yan-jie XIAO Yang |
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| Affiliation: | GUO Hong-bo,LIN Ye,LI Qing-zhang,BIAN Yan-jie,XIAO Yang(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The protein expression and localization of leptin and its long form receptor(OB-Rb) were detected by western blottig methord and a confocal laser scanning microscope.The results showed that leptin protein level was higher in virgin,the lowest in lactation.Then OB-Rb was higher in virgin and pregnancy,lower in lactation,and recovered to the original level in involution. |
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| Keywords: | dairy cow mammary gland leptin OB-Rb |
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