不同品种烤烟多酚含量和组成的差异分析

采用反相高效液相色谱法对2005和2006年河南中部烟区种植的4个烤烟品种初烤烟叶中的6种多酚成分进行了测定。结果表明:①不同烤烟品种的多酚类物质含量存在不同程度的差异,且两年的情况基本相同,中烟100的多酚类物质含量较高,云烟85和云烟87的多酚类物质含量相对较低;②绿原酸及其同分异构体和芸香苷占烤烟多酚类物质的90%以上,是主要的多酚类物质,山奈酚糖苷和莨菪亭含量极低,中烟100的总绿原酸、绿原酸占多酚总量的比例高于NC89和云烟系列,云烟85和云烟87的芸香苷占多酚总量的比例明显高于其它品种;③不同部位多酚含量的差异与烤烟品种也有一定关系。

Difference Analysis of Polyphenol Contents and Proportions in Different Flue-cured Tobacco Cultivars

6 polyphenol compounds in cured leaves of 5 flue-cured tobacco cultivars from central Henan tobacco growing area in 2005 and 2006 were quantitatively determined with reverse phase high performance liquid chromatography.The results showed that: 1) the contents of polypenols in different flue-cured tobacco cultivars varied at different degrees,it was similar in both 2005 and 2006;the polypenols' contents in Zhongyan100 were higher than those in Yunyan85 and Yunyan87;2) chlorogenic acid and its isomer and rutin accounted for more than 90% of total polyphenols in flue-cured tobacco,the contents of kaempferol-3-rutinoside and scopoletin were very low;the proportions of total chlorogenic acid and chlorogenic acid in total polyphenols in Zhongyan100 were higher than those in NC89,Yunyan85 and Yunyan87;while the proportions of rutin in total polyphenols in Yunyan85 and Yunyan87 were obviously higher than those in other cultivars;3) there were some relationships between the differences of polyphenol contents in leaves from different stalk positions and tobacco cultivars.