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一种快速检测呕吐毒素的胶体金试纸条
引用本文:李萌,冯才伟,贾芳芳,冯静,扶胜. 一种快速检测呕吐毒素的胶体金试纸条[J]. 中国酿造, 2013, 32(11): 91-93. DOI: 10.3969/j.issn.0254-5071.2013.11.022
作者姓名:李萌  冯才伟  贾芳芳  冯静  扶胜
作者单位:1.武汉中粮肉食品有限公司,湖北武汉430200;2.北京勤邦生物技术有限公司,北京102206
摘    要:利用胶体金免疫层析法,研制了一种能够快速检测黄豆中呕吐毒素含量的试纸条。通过试验,胶体金试纸条对呕吐毒素的检测限是1μg/g,对玉米赤霉烯酮毒素、黄曲霉毒素B1、赭曲毒素A等无交叉反应,假阳性率和假阴性率均为0,检测时间为10min。试纸条能够准确、可靠、简便、快速地检测黄豆中残留的呕吐毒素,适合大量样品的现场检测。

关 键 词:呕吐毒素  胶体金试纸条  黄豆  快速检测  

Gold immunochromatography assay for rapid detection ofvomitoxin
LI Meng,FENG Caiwei,JIA Fangfang,FENG Jing,FU Sheng. Gold immunochromatography assay for rapid detection ofvomitoxin[J]. China Brewing, 2013, 32(11): 91-93. DOI: 10.3969/j.issn.0254-5071.2013.11.022
Authors:LI Meng  FENG Caiwei  JIA Fangfang  FENG Jing  FU Sheng
Affiliation:1.COFCO Wuhan Meat Product Limited, Wuhan 430200, China; 2.Beijing Kwinbon Biotechnology Company, Beijing 102206, China
Abstract:To establish a rapid assay for detecting vomitoxin in soybean, gold immunochromatography assay was studied. The limit of detection was 1μg/g. The strip was no cross reactions with corn gibberellic ketene toxins, aflatoxin B1 or ochratoxin A. The assay gave no false positive and false negative results. And one could get the results in 10 min. The method was accurate, simple, reliable and convenient to rapidly detect vomitoxin of a lot of soybean samples on the spot.
Keywords:vomitoxin  gold immunochromatography assay strip  soybean  rapid detection
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