| 考马斯亮蓝法测定核桃水溶性蛋白含量的研究 |
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| 引用本文： | 柳荫,吴凤智,陈龙,王晓丹,邱树毅,周鸿翔.考马斯亮蓝法测定核桃水溶性蛋白含量的研究[J].中国酿造,2013,32(12):131-133. |
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| 作者姓名： | 柳荫 吴凤智 陈龙 王晓丹 邱树毅 周鸿翔 |
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| 作者单位： | 贵州大学酿酒与食品工程学院，贵州省发酵工程与生物制药省级重点实验室，贵州贵阳550025 |
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| 基金项目： | 贵州省农业攻关项目（黔科合NZ[2013]3016号） |
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| 摘 要： | 采用考马斯亮蓝G-250染色法对核桃粗蛋白中可溶性蛋白质含量进行了测定，结果表明，蛋白质浓度为10mg/mL~80mg/mL时与吸光度值有良好的线性关系，考马斯亮蓝G-250染色法简单、快速、灵敏，且相对较为准确，是测定低浓度蛋白质含量的有效方法。通过此方法，测得的核桃水溶性蛋白平均含量为8.37%。
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| 关 键 词： | 核桃 蛋白质 考马斯亮蓝 |
Determination of water-soluble protein in walnut by Bradford method |
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| LIU Yin;WU Fengzhi;CHEN long;WANG Xiaodan;QIU Shuyi;ZHOU Hongxiang.Determination of water-soluble protein in walnut by Bradford method[J].China Brewing,2013,32(12):131-133. |
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| Authors: | LIU Yin;WU Fengzhi;CHEN long;WANG Xiaodan;QIU Shuyi;ZHOU Hongxiang |
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| Affiliation: | Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineening, Guizhou University, Guiyang, 550025, China |
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| Abstract: | The soluble protein content in walnut was determined by using coomassie brilliant blue G-250.Result showed there was a good linear relationship between protein concentration and absorbance value within the concentration value of 10mg/mL～80mg/mL.The Bradford method was simple,rapid,sensitive and relatively accurate,which was an effective method for measuring low concentrations protein content.By this method,the measured average protein content of walnut was 8.37％. |
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| Keywords: | walnut protein coomassie brilliant blue （CBB） |
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