| 雨生红球藻转化条件及虾青素积累的研究 |
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| 引用本文： | 张睿钦,顾洪玲,管斌,孔青. 雨生红球藻转化条件及虾青素积累的研究[J]. 中国酿造, 2013, 32(11): 47-50. DOI: 10.3969/j.issn.0254-5071.2013.11.011 |
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| 作者姓名： | 张睿钦 顾洪玲 管斌 孔青 |
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| 作者单位： | 1.淮南师范学院生命科学系，安徽淮南232000；2.中国海洋大学食品科学与工程学院，山东青岛266003 |
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| 基金项目： | 十二五农村领域国家科技计划课题（2013BAD10802-06）;山东省自然科学基金资助项目（2010ZRE29035） |
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| 摘 要： | 在单因素试验基础上，选择转化光照强度、转化温度和转化时间为自变量，虾青素含量为响应值，利用Box-Behnken实验和响应面分析法，对转化条件进行优化。结果表明，在温度29.92℃，pH值10，光照7203.95lx的条件下转化10.6d，虾青素含量可达（33.796±1.556）mg/L，与未优化转化条件时相比，提高了0.99倍。
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| 关 键 词： | 雨生红球藻 转化条件 虾青素 响应面法 |
Transformation conditions of Haematococcus pluvialis and accumulation of astaxanthin |
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| ZHANG Ruiqin,GU Hongling,GUAN Bin,KONG Qing. Transformation conditions of Haematococcus pluvialis and accumulation of astaxanthin[J]. China Brewing, 2013, 32(11): 47-50. DOI: 10.3969/j.issn.0254-5071.2013.11.011 |
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| Authors: | ZHANG Ruiqin GU Hongling GUAN Bin KONG Qing |
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| Affiliation: | 1.Department of Life Sciences, Huainan Normal University, Huainan 232000, China; 2.School of Food Science and Engineering,
Ocean University of China, Qingdao 266003, China |
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| Abstract: | To enhance the transformation efficiency and astaxanthin accumulation, single factor experiment was carried out. Box-Behnken experiments and response surface methodology were employed to optimize transformation conditions. The results showed a distinctive effect of light intensity, temperature and transformation time on astaxanthin accumulation in Haematococcus pluvialis. The optimal transformation conditions were that light intensity 7203.951x, temperature 29.92℃ and pH 10. Astaxanthin content reached （33.79638±1.5561）mg/L which raised 0.99-fold against the control group. |
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| Keywords: | Haematococcus pluvialis transformation conditions astaxanthin response surface methodology （RSM） |
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