温莪术不同溶剂提取物体外抗氧化活性评价

目的:探讨温莪术不同溶剂提取物体外的抗氧化活性。方法:分别用蒸馏水、95%乙醇、乙酸乙酯等不同极性溶剂提取温莪术中活性物质,采用清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟自由基(.OH)、亚铁离子螯合和还原能力等方法考察温莪术不同提取物的抗氧化活性。同时用Folin-Cioeaile法测定不同提取物中的总酚含量,考察总酚含量与抗氧化清除率的关系。结果:DPPH自由基、.OH清除能力及还原能力结果表明95%乙醇及乙酸乙酯提取物在较低的质量浓度显示了较高的清除率;同时总酚含量测定结果显示95%乙醇和乙酸乙酯提取物所含总酚含量较高,这表明这两种溶剂提取物的抗氧化性与其提取物含有较高的酚类密切相关;亚铁离子螯合实验结果显示水提物的螯合效果优于其他溶剂提取物。结论:不同溶剂提取物抗氧化能力差别较大,总酚含量与抗氧化清除率具有相关性。

Antioxidant Activities in vitro of Different Solvent Extracts from Curcuma wenyujin Root Tubers

Objective: To explore the in vitro antioxidant activities of different solvent extracts from the root tubers of C.wenyujin.Methods: The root tubers of C.wenyujin were extracted with solvents of different polarity,such as distilled water,95% ethanol and ethyl acetate,respectively.The resulting extracts were assessed for antioxidant properties,such as DPPH free radical scavenging activity,hydroxyl free radical scavenging activity,ferrous ion-chelating capacity and reducing power.Meanwhile,the total phenol contents of the extracts were determined by the Folin-Ciocaile method and the correlation between total phenol content and antioxidant properties was analyzed.Results: The 95% ethanol and ethyl acetate extracts showed high free radical scavenging activity at low concentrations and had higher total phenol content than the distilled water extract.Therefore,the powerful antioxidant activity of the 95% ethanol and ethyl acetate extracts is immediately linked to their higher total phenol content.However,their ferrous ion-chelating capacity was inferior to that of the distilled water extract.Conclusions: The different solvent extracts from the root tubers of C.wenyujin show a considerable difference in their antioxidant activities and a correlation between their total phenol content and antioxidant properties.