松口蘑糖蛋白联合5-Fu体外对人肝癌细胞Bel-7402的影响及作用机理初探

采用MTT法研究了松口蘑抗肿瘤糖蛋白MTS03与化疗药物5-Fu单独及联合使用对人肝癌细胞Bel-7402的影响及对人正常肝细胞L-02的毒性作用，观察不同浓度配比及不同作用时间对细胞是否具有增效减毒的作用；采用扫描电镜观察MTS03作用后的Bel-7402细胞形态．结果表明，MTS03与5-Fu在较低浓度单独使用对肿瘤细胞无显著差异，但两者联合使用增强了对肿瘤细胞的杀伤作用，而对正常细胞的作用较小，联合使用的q值均大于0．85，说明不存在拮抗作用．扫描电镜观察到凋亡小体的形成，随着剂量升高，凋亡小体增多．这一结果说明两者合用后MTS03增加了5-Fu对肝癌细胞的敏感性，同时降低了两者单一高剂量使用时引起的毒副作用，同时MTS03能诱导Bel-7402细胞膜皱缩和凋亡小体的形成．

Combinational Effects of a Glycoprotein from Tricholoma matsutake with 5-Fu on Human Liver Cancer Cells Bel-7402 in Vitro and the Relevant Mechanisms

MTT assay was used to study the effects of a glycoprotein MTS03 from Tricholoma matsutake combined with 5-Fu on human liver cancer Bel-7402 cells and the cytotoxicity of the drugs against human liver L-02 cells. The preliminary mechanism of MTS03 on cancer cells was also investigated. The results showed that MTS03 significantly decreased the survival level of Bel-7402 cells combined with 5-Fu at low dose, as compared with that using the drugs individually, and it had no or little side effects against human liver L-02 cells. The values of q were larger than 0. 85, they had no antagonistic effect with each other. The cells treated with MTS03 at dosage of 40, 20, 5 g/mL were abnormal, and apoptotic bodies were visualized by microscope and scanning electromicroscope. The result suggested that MTS03 could increase the sensitivity of human liver cancer cells to 5-Fu, and reduce the side effects when using high dosage of either MTS03 or 5-Fu, MTS03 could also cause cell shrinkage with the formation of apoptotic bodies.