Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines |
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Authors: | Chun-qiao Xiao Ru-an Chi Huan He and Wen-xue Zhang |
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Affiliation: | (1) Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha, 410083, China;(2) Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430073, China;(3) Yunnan Phosphate Chemical Group Co. Ltd, Jinning, 650600, China |
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Abstract: | The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines (Hubei, China), were studied
in National Botanical Research Institute’s phosphate (NBRIP) growth medium containing tricalcium phosphate (TCP) as sole phosphorus
(P) source. The strain YC is identified as Stenotrophomonas maltophilia (S. maltophilia) based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The
results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble
P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium
are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis.
It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of
the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source,
respectively, in NBRIP growth medium.
Foundation item: Project(2004CB619201) supported by the Major State Basic Research and Development Program of China; Project(Z200515002) supported
by the Key Project Foundation of the Education Department of Hubei Province, China; Project(GCP200801) supported by the Open
Research Fund of Key Laboratory for Green Chemical Process of Ministry of Education, China; Project(Q200811) supported by
the Youths Science Foundation of Wuhan Institute of Technology, China |
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Keywords: | tricalcium phosphate (TCP) Stenotrophomonas maltophilia (S maltophilia) phosphate mines phosphorus (P) gluconic acid |
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