| EHV-1_(CN)与IL-2基因共表达核酸疫苗诱导产生CTL的观察 |
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| 引用本文： | 郭焱,金宁一,张应玖,夏志平,王宏伟,郭志儒,罗晶,殷震. EHV-1_(CN)与IL-2基因共表达核酸疫苗诱导产生CTL的观察[J]. 中国生物制品学杂志, 2001, 0(2) |
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| 作者姓名： | 郭焱 金宁一 张应玖 夏志平 王宏伟 郭志儒 罗晶 殷震 |
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| 基金项目： | 国家自然科学基金（3977066），国家杰出青年基（39825119）资助 |
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| 摘 要： | 目的 观察ＨＩＶ－１ＣＮ ｇｐ１２０基因与ＩＬ－２基因共表达核酸疫苗质粒ｐＧＰＩＬ－２诱导产生ＣＴＬ。方法 脂质体介导共表达中国流行株ＨＩＶ－１Ｇａｇ－ｇｐ１２０基因与ＩＬ－２基因的核酸疫苗质粒ｐＧＩＬ－２转染ＢＨＫ－２１细 胞，以间接免疫光法鉴定其表达。取ｐＧＰＩＬ－２免疫鼠脾细胞，检测ｐＧＰＩＬ－２诱导的细胞毒性Ｔ细胞杀伤活性。 结果ｐＧＰＩＬ－２可有效地诱导ＣＩＬ的产生，并可杀伤ＨＩＶ－１ＣＮ嵌合基因转染的靶细胞。结论 为进一步设计中国流行株ＨＩＶ－１核酸疫苗提供了重要实验依据。
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| 关 键 词： | gp120基因 IL-2基因 细胞毒性T细胞 |
Cytotoxic T Lymphocyte Induced with DNA Vaccine Prepared by Co-expression of HIV-1_(CN) gp 120 and IL-2 Genes |
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| GUOYan,JIN Ningyi,ZHANG Yingjin et al. Cytotoxic T Lymphocyte Induced with DNA Vaccine Prepared by Co-expression of HIV-1_(CN) gp 120 and IL-2 Genes[J]. Chinese Journal of Bilogicals, 2001, 0(2) |
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| Authors: | GUOYan JIN Ningyi ZHANG Yingjin et al |
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| Abstract: | Objective To observe the cytotoxic T lymphocyte(CTL)induced with the DNA vaccine prepared by the co-expression of HIV-1CN gp 120 and IL-2 genes. Methods The plasmid pGPIL-2 for the coexpression of HW-1 Gag-gp 120 and IL-2 genes was transfected to BHK-21 cells under the mediation of liposome, and the expressed product was identified by indirect immumofluorescence method. Murine splenocytes were immunized with the pGPIL-2, and the CFL killing activity of the mice was detected. Results The CTL killing activity to the target cells transfected with HIV-1CN chimeric gene was effectively induced by pCPIL-2. Conclusion The study provides an important basis for further developing the DNA vaccine against the current HIV-1 strain in China. |
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| Keywords: | gp120 IL-2 CTL |
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