高效液相色谱法测定鸭血制品中甲醛含量

建立鸭血制品中甲醛的高效液相色谱测定法。以衍生液2,4-二硝基苯肼乙腈溶液-水（1∶1，v/v）的混合溶液为提取剂对样品进行提取，Waters SunFireC18（4.6 mm×250 mm，5 μm）色谱柱进行分离，流动相为甲醇-水（65∶35，v/v），流速1.0 mL/min，在波长365 nm处以紫外检测器检测，外标法定量。结果表明，甲醛在线性范围0.4～6.0 μg/mL内，相关系数为0.999 7，最低检出限为0.2 mg/kg，精密度（相对标准偏差）小于5%，加标回收率为85.1%～92.7%。本方法操作简便、灵敏度高、分离度好，适用于鸭血中甲醛含量的测定。

Determination of Formaldehyde in Duck Blood Products by HPLC

A high performance liquid chromatography (HPLC) method for the determination of formaldehyde in duck blood
products is presented in this paper. The formaldehyde in sample was extracted and derivatized with a mixture of 2,4-dinitrophenylhydazine
dissolved in acetonitrile (300 mg/500 mL) and water (1:1, by volume), and separated on a Waters SunFire
C18 column (4.6 mm × 250 mm, 5 μm), using a mobile phase composed of methanol and water (65:35, by volume) at a
flow rate of 1.0 mL/min. The compound was detected by UV detector at 365 nm and then quantified by external calibration
method. The results showed that the proposed method displayed good linear relationship over the concentration range of
0.4–6.0 μg/mL, with a correlation coefficient of 0.999 7. The limit of detection (LOD) was 0.2 mg/kg, and the precision
expressed as relative standard deviation (RSD) for 6 replication determinations was blew 5%. The average recoveries
for blank spiked samples were in the range of 85.1%–92.7% (n = 3). This method is simple and convenient with good
separation for the analysis of formaldehyde in duck blood products.