| 响应面法优化提高发酵桑叶茶中1-脱氧野尻霉素含量工艺 |
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| 引用本文： | 肖洪,黄先智,沈以红,丁晓雯,秦樱瑞,曾艺涛,杨娟,丁顺杰,商桑.响应面法优化提高发酵桑叶茶中1-脱氧野尻霉素含量工艺[J].食品科学,2014,35(10):46-51. |
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| 作者姓名： | 肖洪 黄先智 沈以红 丁晓雯 秦樱瑞 曾艺涛 杨娟 丁顺杰 商桑 |
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| 作者单位： | 1.西南大学食品科学学院，重庆市农产品加工重点实验室，重庆 400715；;
2.家蚕基因组生物学国家重点实验室，重庆 400715 |
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| 基金项目： | 国家现代农业(蚕业)产业技术体系建设专项(CARS-22-ZJ0503);重庆市科委应用技术项目(cstc,2012gg-yyjs80022) |
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| 摘 要： | 为优化提高发酵桑叶茶中1-脱氧野尻霉素含量工艺,采用响应面分析法,以发酵温度、发酵时间及接种量为自变量,1-脱氧野尻霉素含量为响应值,设计三因素三水平响应面回归分析。结果表明:提高发酵桑叶茶中1-脱氧野尻霉素含量工艺的最佳工艺条件为发酵温度30℃、发酵时间5.6 h、黑曲霉∶日本根霉∶绿色木霉=2∶1∶2菌液接种量3.75×107 CFU/100 g,在此条件下发酵桑叶茶中1-脱氧野尻霉素含量为133.882 mg/100 g。
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| 关 键 词： | 发酵 桑叶茶 1-脱氧野尻霉素 响应面分析 |
Optimization of Fermentation Conditions for Enhanced 1-Deoxynojirimycin Content in Fermented Mulberry Leaf Tea by Response Surface Methodology |
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| XIAO Hong,HUANG Xian-zhi,SHEN Yi-hong,DING Xiao-wen,QIN Ying-rui,ZENG Yi-tao,YANG Juan,DING Shun-jie,SHANG Sang.Optimization of Fermentation Conditions for Enhanced 1-Deoxynojirimycin Content in Fermented Mulberry Leaf Tea by Response Surface Methodology[J].Food Science,2014,35(10):46-51. |
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| Authors: | XIAO Hong HUANG Xian-zhi SHEN Yi-hong DING Xiao-wen QIN Ying-rui ZENG Yi-tao YANG Juan DING Shun-jie SHANG Sang |
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| Affiliation: | 1. Chongqing Key Laboratory of Agricultural Product Processing, College of Food Science, Southwest University,;
Chongqing 400715, China; 2. State Key Laboratory of Silkworm Genome Biology, Chongqing 400715, China |
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| Abstract: | Response surface methodology was used to optimize the fermentation conditions for producing fermented
mulberry leaf tea with higher 1-deoxynojirimycin (1-DNJ) content. The 1-DNJ content was investigated with respect to
fermentation temperature, fermentation time and inoculum amount by response surface regression analysis. The optimum
fermentation conditions that provided a 1-DNJ content of 133.882 mg/100 g dry weight of fermented mulberry leaf tea were
determined as 30 ℃, 5.6 h, a ratio of Aspergillus niger to Rhizopus japonicus Vuillemin to Trichoderma viride of 2:1:2, and
an inoculum amount of 3.75×107 CFU/100 g. |
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| Keywords: | fermentation mulberry leaves 1-deoxynojirimycin (1-DNJ) response surface analysis |
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