鲫鱼卵唾液酸糖蛋白对去卵巢大鼠骨质疏松症的改善作用

目的:从鲫鱼卵中提取唾液酸糖蛋白,研究其对去卵巢大鼠骨质疏松症的改善作用,并探究其作用机理。方法:采用切除大鼠双侧卵巢的方法建立骨质疏松症大鼠模型,灌胃鲫鱼卵唾液酸糖蛋白(400 mg/(kg·d))90 d后,分别检测大鼠尿液骨吸收指标(脱氧吡啶啉、钙、磷)、血清骨吸收指标(抗酒石酸酸性磷酸酶、组织蛋白酶K)、血清骨生成指标(骨源性碱性磷酸酶、骨钙素、Ⅰ型前胶原羧基端前肽)以及血清骨保护素(osteoprotegerin,OPG)、核因子κB受体活化因子(receptor activator for nuclear factor-κB,RANK)、核因子κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)含量。结果:鲫鱼卵唾液酸糖蛋白能显著降低骨质疏松症大鼠尿液脱氧吡啶啉(P0.01)、钙(P0.01)、磷(P0.01)含量和血清抗酒石酸酸性磷酸酶(P0.01)、组织蛋白酶K(P0.01)活性,防止大鼠骨吸收;显著降低血清骨源性碱性磷酸酶(P0.01)活性和骨钙素(P0.01)、Ⅰ型前胶原羧基端前肽(P0.01)含量,抑制大鼠高骨转换速率;显著上调OPG(P0.01)含量,下调RANKL(P0.01)含量,降低RANKL/OPG比值,抑制破骨细胞增殖分化,降低骨吸收。结论:鲫鱼卵唾液酸糖蛋白具有改善去卵巢大鼠骨质疏松症的作用,其作用机理可能与下调RANKL/OPG比值有关。

Improvement Effect of Sialoglycoprotein from Crucian Carp (Carassius auratus) Spawn in Ovariectomized Female Rats with Osteoporosis

Objective: To evaluate the improvement effect of sialoglycoprotein from crucian carp (Carassius auratus)
spawn on osteoporosis in ovariectomized female rats and to explore the underlying mechanism. Methods: The animal
model of osteoporosis was established by removing bilateral ovaries. The rats were intragastrically administered with the
sialoglycoprotein at a daily dosage of 400 mg/(kg·d) for 90 days. At the end of the experiment, serum and urine indexes
indicating bone absorption and formation, and osteoprotegerin (OPG) level, receptor activator for nuclear factor-κB (RANK)
and receptor activator for nuclear factor-κB ligand (RANKL) in serum were determined. Results: Sialoglycoprotein from
crucian carp spawn showed a suppressive effect on the contents of deoxypyridinoline, calcium and phosphorus in urine and
the activity of tartrate-resistant acid phosphatase and cathepsin-K in serum associated with bone absorption, the activity of
bone alkaline phosphatase and the contents of osteocalcin, propeptide carboxy-terminal procollagen in serum associated with
bone formation. Meanwhile, it also up-regulated the contents of OPG, down-regulated the contents of RANKL and decreased the
ratio of RANKL to OPG. Therefore, the sialoglycoprotein could prevent bone absorption in ovariectomized rats with osteoporosis
and inhibit its high bone turnover rate. Conclusion: Sialoglycoprotein from crucian carp spawn could prevent osteoporosis of
ovariectomized rats, and its functional mechanism is probably related to the decrease of RANKL/OPG ratio.