高效液相色谱法测定紫苏中5 种有机酸和黄酮的含量

目的：建立以甲醇溶液为萃取剂，结合超声辅助萃取，利用高效液相色谱法同时分离测定紫苏中
的咖啡酸、阿魏酸、迷迭香酸、木犀草素和芹菜素5 种有效成分的方法。方法：采用Phenomenex C18色谱柱
（250 mm×4.6 mm，5 μm）；流动相为甲醇-0.1%冰醋酸溶液，采用梯度洗脱程序；流速1 mL/min；紫外检测波
长320 nm；柱温35 ℃。结果：该方法对咖啡酸、阿魏酸、迷迭香酸、木犀草素和芹菜素分别在0.001 22～61.00
（r=0.999 99）、0.001 06～53.00（r=0.999 99）、0.001 28～64.00（r=0.999 91）、0.001 20～60.00（r=0.999 94）、
0.001 12～56.00 μg/mL（r=0.999 95）范围内线性关系良好，相关系数（r）均大于0.999 91，检出限（RSN=3）依次
是：0.101 9、0.392 6、0.355 9、0.286 2、0.202 5 ng/mL；样品回收率为95.79%～101.41%。结论：本法操作简单快
速、定量准确、灵敏度高、成本低、且对环境友好，为紫苏中咖啡酸、阿魏酸、迷迭香酸、木犀草素和芹菜素的分
离检测提供了一个有效的方法。

Determination of Five Organic Acids and Flavonoids in Perilla frutescens by High Performance Liquid Chromatography

Objective: A simple high performance liquid chromatography (HPLC) method has been developed for the
simultaneous quantification of caffeic acid, ferulic acid, rosmarinic acid, luteolin and apigenin from Perilla frutescens using
methanol as extractant with ultrasonic-assisted extraction. Methods: The five compounds were isolated by using a Phenomenex
C18 column by gradient elution, and the mobile phase was methanol-0.1% acetic acid solution with a flow rate of 1.0 mL/min.
The UV detection wavelength was performed at 320 nm. Results: The linear ranges for caffeic acid, ferulic acid, rosmarinic
acid, luteolin and apigenin were determined to be 0.001 22–61.00, 0.001 06–53.00, 0.001 28–64.00, 0.001 20–60.00 and
0.001 12–56.00 μg/mL, respectively, and the correlation coefficients were 0.999 99, 0.999 99, 0.999 91, 0.999 94 and 0.999 95,
respectively. The limits of detection (LOD) were 0.101 9, 0.392 6, 0.355 9, 0.286 2 and 0.202 5 ng/mL, respectively. The
average recoveries of these compounds in spiked real samples were in the range of 95.79%–101.41%. Conclusion: The
proposed method is simple, precise, specific, sensitive and accurate. It provides a new and scientific means for routine quality
control of Perilla frutescens.