超高效液相色谱-质谱联用测定啤酒中果糖、葡萄糖的含量

建立啤酒中微量果糖和葡萄糖的超高效液相色谱-四极杆质谱联用测定方法。该方法中样品经过滤后直接上机测试，用Waters Acquity色谱柱（2.1 mm×150 mm，1.7 μm）进行分离，流动相采用水∶乙腈（10∶90，V/V），以电喷雾离子源负离子多反应监测模式进行检测。该方法测定果糖的线性范围为1.25～30 mg/L，线性相关系数R2=1，检出限为0.05 mg/L；葡萄糖的线性范围为2～50 mg/L，线性相关系数R2=0.999 9，检出限为0.49 mg/L。将方法用于实际样品检测，结果灵敏、准确、重现性好。

Determination of Fructose and Glucose in Beers by Liquid Chromatography-Mass Spectrometry

A liquid chromatography-mass spectrometry (LC-MS) method was developed for the determination of micro
amounts of fructose and glucose in beers. In this method, samples were detected directly after filtering. The chromatographic
separation was achieved on a Waters Acquity column (2.1 mm×150 mm, 1.7 μm), with a mobile phase composed of water
and acetonitrile (10:90, V/V). The mass spectrometer was operated in the negative mode using MRM．The developed
calibration curves displayed good linearity over a concentration range of 1.25 to 30 mg/L with a correlation coefficient equal
to 1 for fructose, and 2 to 50 mg/L with a correlation coefficient equal to 0.999 9 for glucose. The limits of detection (LOD)
were 0.05 and 0.49 mg/L respectively. This method has been successfully used for the determination of fructose and glucose
in beers with satisfactory results.