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| 重组酶FLP在弱氧化葡糖杆菌中的表达及应用 | |
| 引用本文： | 刘静文,李天明,杜红燕,冯惠勇. 重组酶FLP在弱氧化葡糖杆菌中的表达及应用[J]. 食品科学, 2014, 35(11): 204-208. DOI: 10.7506/spkx1002-6630-201411041 |
| 作者姓名： | 刘静文 李天明 杜红燕 冯惠勇 |
| 作者单位： | 河北科技大学生物科学与工程学院，河北 石家庄 050000 |
| 摘 要： | |
| 关 键 词： | 弱氧化葡糖杆菌 FLP/FRT 位点特异性重组 启动子 基因敲除 |
Expression and Application of Recombinase FLP in Gluconobacter suboxydans |
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| LIU Jing-wen,LI Tian-ming,DU Hong-yan,FENG Hui-yong. Expression and Application of Recombinase FLP in Gluconobacter suboxydans[J]. Food Science, 2014, 35(11): 204-208. DOI: 10.7506/spkx1002-6630-201411041 | |
| Authors: | LIU Jing-wen LI Tian-ming DU Hong-yan FENG Hui-yong |
| Affiliation: | College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, China |
| Abstract: | In this paper, the broad-host vector pBB1MCS-5 was used to construct an expression vector for flippase recombination enzyme (FLP). The expression vector was transformed into Gluconobacter suboxydans, and the positive transformants were screened. The results of validation using a two-step RT-PCR method showed that flp was transcribed and expressed in the host cell. The pBBR1MCS-psldh-flp recombinant plasmid was transformed into tetracyclic-resistant JGDH－ mutant strains, and PCR verification showed that the sites with FTR resistance marker had been effectively removed. Recombination FLP expression in Gluconobacter suboxydans provides a method that circularly uses the selectable marker gene to knock out or replace multiple locus genes. |
| Keywords: | Gluconobacter suboxydans FLP/FRT site-specific recombination promoter gene knock-out |
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