鮟鱇鱼皮胶原蛋白肽的抗氧化活性

选取铁离子还原体系和3 种自由基体系为指标，研究鮟鱇鱼皮胶原蛋白肽体外清除自由基效果和抗氧化作用。采用超滤膜分离鮟鱇鱼皮胶原蛋白肽组分，筛选出体外清除•OH活性最好的组分，再建立小鼠衰老模型，将分离得到活性最好的组分采用灌胃法，测定小鼠皮肤中超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）及丙二醛（malondialdehyde，MDA）和羟脯氨酸（hydroxyproline，Hyp）的含量，研究鮟鱇鱼皮胶原蛋白肽体内抗氧化和清除自由基作用。结果表明：鮟鱇鱼皮胶原蛋白肽具有较强的还原能力且对O－2•、DPPH自由基、•OH具有清除效果，质量浓度为10 mg/mL时对•OH、DPPH自由基、O－2•的清除率和对Fe3+ 的还原能力分别为70.48%、68.78%、42.53%和0.676；分子质量小于2 000 D的鮟鱇皮胶原蛋白肽组分对•OH具有较好的清除效果，IC50为15.7 mg/mL；剂量为100 mg/（kg•d）时，显著提高了皮肤中SOD、GSH-Px、CAT的活性，较模型组分别增加20.9%、41.3%和58.1%，且显著抑制MDA的形成。

Antioxidant Activity of Collagen Peptides from Lophius litulon Skin

A ferric ion-reducing system and three free radical systems were used to evaluate the radical-scavenging capacity
and antioxidant activity of collagen peptides from Lophius litulon skin in vitro. The peptide components were separated by
ultrafiltration and the fraction with the highest hydroxyl radical scavenging activity in vitro was obtained. In addition, aged
mouse models were constructed and determined for SOD, CAT, GSH-Px, MDA and Hyp contents in the skin after oral
administration of the screened fraction. The results showed that Lophius litulon skin collagen peptides had strong reducing
power and superoxide anion, DPPH and hydroxyl radical scavenging capacity in vitro. When the sample concentration was
10 mg/mL, the scavenging rates of hydroxyl, DPPH and superoxide anion radicals and the ferric ion reducing power were
70.48%, 68.78%, 42.53% and 0.676, respectively. The fraction with relative molecular weight less than 2 000 D had stronger
hydroxyl radical scavenging activity, and its IC50 was 15.7 mg/mL. The activities of SOD, GSH-Px and CAT in skin were
increased by respectively 20.9%, 41.3% and 58.1% at the dose of 100 mg/(kg·d) when compared with the model group.
Moreover the generation of MDA was significantly inhibited.