蔗渣制备低聚木糖溶液的脱色脱盐工艺及其组分分析

利用活性炭结合阴阳离子交换树脂吸附技术研究甘蔗渣制备低聚木糖溶液的脱色脱盐工艺，并采用高效液相色谱分析精制后的低聚木糖溶液组分。结果表明：活性炭对低聚木糖溶液最佳脱色工艺为活性炭添加量质量分数1%、反应温度60 ℃、吸附时间1 h，在该条件下溶液脱色率为80.25%、还原糖保留率为98.70%。通过对7 种不同型号的树脂进行筛选，确定选用001×7和D301树脂串联、V（001×7）∶V（D301）=2∶1、流速254 mL/h时，离子交换树脂对低聚木糖脱盐效果最佳。经过活性炭和离子交换树脂共同脱色脱盐，低聚木糖溶液的最终脱色率为92.4%、脱盐率为79.2%，溶液接近中性（pH 7.4）。高效液相色谱法分析确定低聚木糖水解得到的单糖主要为木糖，还含有少量的甘露糖和葡萄糖，其中木糖占所有单糖的88.9%；低聚木糖溶液主要为木二糖和木三糖，还含有少量的木糖和木五糖。

Decolorization,Demineralization and Monosaccharide Composition of Xylooligosaccharides from Sugarcane Bagasse

Decolorization and demineralization of xylooligosaccharides (XOs) from sugarcane bagasse were investigated
by activated charcoal combined with ion exchange resin adsorption. The monosaccharide composition of XOs was analyzed
by high performance liquid chromatography (HPLC). Results showed that the optimum decolorization conditions for
XOs were addition of 1% activated charcoal followed by adsorption at 60 ℃ for 1 h, resulting in a decolorization rate of
80.25% and a retention rate of reducing sugar of 98.7%. Through a comparative analysis of seven different types of resins,
sequential chromatography on 001×7 and D301 with a volume ratio of 2:1 at a flow rate of 254 mL/h was chosen for
the best demineralization of XOs. The decolorized and demineralized XOs exhibited a decolorization rate of 92.4% and a
demineralization rate of 79.2%, and were nearly neutral (pH 7.4). HPLC analysis showed that the major monosaccharide
from acid hydrolysates of purified XOs, accounting for 88.9% of the total monosaccharides, and small amounts of mannose
and glucose were detected as well. The XOs obtained in this study mainly contained xylobiose and xylotriose, together with
a small amount of xylose and xylopentaose.