首页 | 本学科首页   官方微博 | 高级检索  
     

响应面法优化酶辅助闪式提取二氢槲皮素工艺
引用本文:柴婧,付警辉,张卫鹏,郑毅男,刘文丛. 响应面法优化酶辅助闪式提取二氢槲皮素工艺[J]. 食品科学, 2014, 35(10): 93-97. DOI: 10.7506/spkx1002-6630-201410017
作者姓名:柴婧  付警辉  张卫鹏  郑毅男  刘文丛
作者单位:吉林农业大学中药材学院,吉林 长春 130118
基金项目:吉林省科技厅项目(20115064)
摘    要:目的:利用响应面法对纤维素酶辅助闪式提取二氢槲皮素的工艺条件进行优化。方法:以二氢槲皮素的提取率为指标,在单因素试验的基础上,应用Box-Behnken试验设计,对影响提取率的条件(乙醇体积分数、加酶量和液料比)进行优化。结果:最佳提取条件为乙醇体积分数60.93%、加酶量3.48 mg/g、液料比103.64∶1(mL/g)、提取时间120 s。结论:闪式提取操作简便、节能、省时,可应用于二氢槲皮素大规模生产。

关 键 词:长白落叶松  二氢槲皮素  纤维素酶  闪式提取  响应面分析法  

Optimization of Cellulase-Assisted Extraction of Dihydroquercetin by Response Surface Methodology
CHAI Jing,FU Jing-hui,ZHANG Wei-peng,ZHENG Yi-nan,LIU Wen-cong. Optimization of Cellulase-Assisted Extraction of Dihydroquercetin by Response Surface Methodology[J]. Food Science, 2014, 35(10): 93-97. DOI: 10.7506/spkx1002-6630-201410017
Authors:CHAI Jing  FU Jing-hui  ZHANG Wei-peng  ZHENG Yi-nan  LIU Wen-cong
Affiliation:College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China
Abstract:Objective: To optimize the celluase-assisted extraction of dihydroquercetin from Larix olgensis Henry var.
koreana Nakai wood chips by response surface methodology (RSM). Methods: Dihydroquercetin was extracted with ethanol
in a herbal blitzkrieg extractor from pine wood chips pre-hydrolyzed with celluase. On the basis of the results of single-factor
experiments, a Box-Behnken design was performed to optimize three extraction conditions including ethanol concentration,
enzyme dosage and liquid-to-solid ratio. Results: The optimal extraction conditions were determined as hydrolysis with an
enzyme dosage of 3.48 mg/g and subsequent extraction for 120 s with 60.93% ethanol in water at a liquid-to-solid ratio of
103.64:1 (mL/g). Conclusion: The proposed extraction procedure can provide a simple, energy-saving and time-saving way
for massive production of dihydroquercetin.
Keywords:Larix olgensis Henry var. koreana Nakai  dihydroquercetin  cellulase  extraction  response surface methodology (RSM)  
本文献已被 CNKI 等数据库收录!
点击此处可从《食品科学》浏览原始摘要信息
点击此处可从《食品科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号