| 液相色谱-四极杆-飞行时间质谱法筛查食品中6 种人工合成甜味剂 |
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| 引用本文： | 王建华,杨君,刘琳,颜冬云,李立,刘靖靖.液相色谱-四极杆-飞行时间质谱法筛查食品中6 种人工合成甜味剂[J].食品科学,2014,35(8):181-186. |
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| 作者姓名： | 王建华 杨君 刘琳 颜冬云 李立 刘靖靖 |
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| 作者单位： | 1.山东出入境检验检疫局检验检疫技术中心，山东 青岛 266002；2.青岛大学化学科学与工程学院
（环境科学与工程学院），山东 青岛 266071；3.中国检验检疫科学研究院，北京 100123 |
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| 基金项目： | 质检公益性行业科研专项(2012424012-2) |
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| 摘 要： | 应用高效液相色谱-四极杆-飞行时间质谱建立固体样品中甜味剂的筛查。样品经前处理后采用Eclispe XDB-C18色谱柱，以0.1%甲酸-10 mmol/L甲酸铵溶液-甲醇为流动相，梯度洗脱，电喷雾负离子模式分析检测。在 全扫描采集模式下，以准分子离子峰的峰面积定量，以化合物精确分子质量定性。各化合物在0.02～2.0 mg/L范围 内均呈现良好的线性关系，相关系数均大于0.998。样品平均添加回收率为74.5%～120.1%，相对标准偏差均小于 10.4%。本方法可满足现行法规的限量要求。
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| 关 键 词： | 液相色谱-四极杆飞行时间串联质谱 人工合成甜味剂 筛查 食品 |
Analysis of 6 Synthetic Sweeteners in Foods by Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry |
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| WANG Jian-hua,YANG Jun,LIU Lin,YAN Dong-yun,LI Li,LIU Jing-jing.Analysis of 6 Synthetic Sweeteners in Foods by Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry[J].Food Science,2014,35(8):181-186. |
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| Authors: | WANG Jian-hua YANG Jun LIU Lin YAN Dong-yun LI Li LIU Jing-jing |
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| Affiliation: | 1. Technical Center of Inspection and Quarantine, Shandong Entry-Exit Inspection Quarantine Bureau, Qingdao 266002, China;
2. College of Chemical Engineering, Qingdao University, Qingdao 266071, China;
3. Chinese Academy of Inspection and Quarantine, Beijing 100123, China |
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| Abstract: | A new method was established for the determination of 6 synthetic sweeteners (sodium saccharin, cyclamate,
acesulfame-K, aspartame, neotame, and sucralose) in food samples by using liquid chromatography-quadrupole-time
of flight mass spectrometry (LC-Q-TOF-MS). The extract was measured directly by LC-Q-TOF-MS with electrospray
ionization in the negative mode. The compounds were separated by an Eclispe XDB-C18 column with acetonitril-10 mmol/L
ammonium formate-0.1% formic acid as the mobile phase. A detailed fragmentation study for these 6 synthentic sweeteners
was carried out by time-of-flight. The 6 compounds behaved linearly in the concentration range of 0.02–2.0 mg/L, with a
correlation coefficient > 0.998. The recoveries were 74.5%–120.1%, with relative standard deviation below 10.4%. The
method illustrated is suitable for routine qualitative and quantitative analyses of synthetic sweeteners in food samples. |
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| Keywords: | liquid chromatography quadrupole-time of flight mass spectrometry artificial sweeteners screening food |
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