女贞内生真菌清除自由基活性成分的分离纯化

采用组织块分离法对女贞的内生真菌进行分离，对分离到的菌株进行清除1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl，DPPH）自由基活性研究，并对活性较强菌株代谢产物中的清除自由基成分进行分离纯化。从女贞的叶片、茎、根、枝条等组织共分离出101 株内生真菌，研究发现NZ-18菌株清除自由基活性最强，并采用液相色谱法对NZ-18发酵液中清除自由基活性成分进行制备。分离到一种活性较强的化合物1，经高效液相色谱法（high performance liquid chromatography，HPLC）法和薄层色谱（thin layer chromatography，TLC）法纯度验证，发现该化合物为纯品化合物经活性验证发现化合物1在样品质量浓度为0.5 mg/mL时对0.2 mg/mL的DPPH自由基清除率为94.88%。结果表明，女贞内生真菌中存在大量的清除自由基活性菌株。

Separation and Purification of Free Radical Scavenging Bioactive Components fromEndophytic Fungi in Ligustrum lucidum

Endophytic fungi were isolated from Ligustrum lucidum by tissue separation and their 1,1-diphenyl-2-
picrylhydrazyl (DPPH) radical scavenging activity was studied. Metabolite components with high DPPH radical scavenging
activity were purified. One hundred and one strains of endophytic fungi were isolated from leaves, stems, roots and branches.
It was found that NZ-18 strain had the highest DPPH radical scavenging activity and an active component was prepared from
its fermentation metabolites by liquid chromatography, which was identified as a pure compound by HPLC and TLC and named
as compound 1. The compound I at a concentration of 0.5 mg/mL could scavenge 94.88% of DPPH at 0.2 mg/mL. The present study
indicated that there were many endophytic strains from Ligustrum lucidum whose metabolites could scavenge DPPH free radical.