桦褐孔菌多糖脱色方法及其成分分析

对桦褐孔菌多糖的脱色方法和单糖的组成进行研究。首先考察活性炭粉、过氧化氢、壳聚糖、聚酰胺层析柱的脱色效果。经脱蛋白、脱色后的多糖进行1-苯基-3-甲基-5-吡唑啉酮衍生化，采用高效液相色谱法分析单糖组成。4 种脱色方法对桦褐孔菌多糖均有效果，活性炭和聚酰胺层析柱脱色效果明显优于过氧化氢和壳聚糖脱色法，聚酰胺层析柱脱色是较好的方法，其脱色率为89.3%、多糖保留率为91.7%。结果表明：桦褐孔菌多糖粗品主要由甘露糖、鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖组成，其物质的量比为2.13∶1.36∶7.01∶2.98∶1∶1.78。

Decolorization and Monosaccharide Composition Analysis of Polysaccharides from Inonotus obliguus

In the present work, activated carbon powder, H2O2, chitosan, and polyamide column chromatography were
compared for their effects in decolorizing Inonotus obliguus polysaccharides, and the decolorized polysaccharides were
analyzed for monosaccharide composition by high performance liquid chromatography (HPLC) after derivatization with
1-pheny-3-methyl-5-pyrazolone. All decolorants were able to decolorize Inonotus obliguus polysaccharides, with activated
carbon powder and polyamide column chromatography being more significantly effective than the other decolorants. The
decolorization efficiency of polyamide column chromatography was 89.3% while retaining 91.7% of polysaccharides. The
decolorized polysaccharides were mainly composed of mannose, rhamnose, glucose, galactose, xylose, and arabinose with a
molar ratio of 2.13:1.36:7.01:2.98:1:1.78.