氯氰菊酯降解菌的分离鉴定及其降解底物谱研究

采用富集培养法，从长期受氯氰菊酯农药污染的果园土壤中，分离筛选得到1 株可降解氯氰菊酯的菌株N-02。经形态、生理生化特征及16S rDNA序列分析，初步鉴定为点状气单胞菌（Aeromonas punctata）。菌株N-02在35 ℃、pH 7.5、氯氰菊酯质量浓度为20 mg/L的LB培养基中培养96 h，对氯氰菊酯的降解率达到62.31%。该菌株N-02对拟除虫菊酯的降解谱较宽，培养96 h对20 mg/L高效氯氰菊酯、溴氰菊酯和氟氯氰菊酯降解率分别达到40.17%、33.26%和30.45%。

Isolation,Identification, Degradation Characteristics of a Bacterial Strain Capable of Degrading Cypermethrin

A bacterial strain named N-02 capable of degrading cypermethrin was isolated from long-term cypermethrin
contaminated orchard soil by using enrichment culture. Based on the morphology, physio-biochemical characteristics, and
16S rDNA sequence analysis, strain N-02 was identified as Aeromonas punctata. The degradation rate of cypermethrin was
62.31% when the strain were cultured at 35 ℃ for 96 h in LB medium containing 20 mg/L cypermethrin at pH 7.5. A wide
degradation spectrum of pyrethroid by strain N-02 was observed, and the degradation rates of β-cypermethrin, deltamethrin
and fluoride cypermethrin after 96 h were 40.17%, 33.26% and 30.45%, respectively.