| 乳酸乳球菌KLDS4.0325的B族维生素生物合成途径分析 |
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| 引用本文： | 杨晓春,王玉堂,霍贵成. 乳酸乳球菌KLDS4.0325的B族维生素生物合成途径分析[J]. 食品科学, 2014, 35(7): 86-89. DOI: 10.7506/spkx1002-6630-201407018 |
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| 作者姓名： | 杨晓春 王玉堂 霍贵成 |
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| 作者单位： | 东北农业大学 乳品科学教育部重点实验室，黑龙江 哈尔滨 150030 |
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| 基金项目： | 国家自然科学基金面上项目(31171717);国家高技术研究发展计划(863计划)项目(2012AA022108);食品安全与营养协同创新中心项目 |
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| 摘 要： | 为了探究乳酸乳球菌KLDS4.0325的B族维生素合成潜力，利用各类B族维生素生物合成途径的相关蛋白序列针对该菌株的氨基酸序列进行同源性搜索，并与其他9 株乳酸乳球菌的叶酸生物合成途径进行比较分析。结果表明：与参考菌株相比，乳酸乳球菌KLDS4.0325具有较为完整的叶酸和核黄素合成途径编码基因，在基因水平上可以有效合成叶酸和核黄素，具有相当大的工业潜能。
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| 关 键 词： | 乳酸乳球菌KLDS4.0325 叶酸 核黄素 B族维生素 合成途径 |
Biosynthetic Pathways of B-Group Vitamins in Lactococcus lactis subsp. lactis KLDS4.0325 |
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| YANG Xiao-chun,WANG Yu-tang,HUO Gui-cheng. Biosynthetic Pathways of B-Group Vitamins in Lactococcus lactis subsp. lactis KLDS4.0325[J]. Food Science, 2014, 35(7): 86-89. DOI: 10.7506/spkx1002-6630-201407018 |
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| Authors: | YANG Xiao-chun WANG Yu-tang HUO Gui-cheng |
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| Affiliation: | Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | In order to exploit the potential of Lactococcus lactis subsp. lactis KLDS4.0325 for synthesizing B-groupvitamins, the sequences of proteins involved in the biosynthetic pathways of all kinds of B-group vitamins were used toperform a BLASTp search against the amino acid sequence of L. lactis KLDS4.0325, and comparative analysis with other 9L. lactis strains. Results showed that compared with the reference strains, L. lactis KLDS4.0325 possessed relativelycomplete genes encoding proteins involved in the biosynthetic pathways of folate and riboflavin, could efficiently synthesizefolate and riboflavin at the genetic level. Thus this strain will have a number of potential applications. |
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| Keywords: | L. lactis KLDS4.0325 folate riboflavin B-group vitamin biosynthetic pathways |
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