植物激素对纳塔尔链霉菌发酵产纳他霉素的影响

以纳塔尔链霉菌（Streptomyces natalensis）为发酵菌种，在摇瓶培养基中添加植物激素，通过单因素试验初筛，获得能够有效提高纳他霉素的产量的植物激素；采用Box-Behnken试验设计建立数学模型，进行响应面分析优化确定最优组合。结果表明：吲哚乙酸、赤霉素和6-苄氨基嘌呤均能够有效提高纳他霉素的产量，在5.5 mg/L吲哚乙酸、14.8 mg/L赤霉素和20.5 mg/L 6-苄氨基嘌呤的条件下发酵120 h时，发酵液中纳他霉素的发酵产量可达到1.805 g/L，为对照组的1.63 倍。

Influences of Plant Hormones on the Natamycin Production of Streptomyces natalensis

Screening of five plant hormones was carried out to find out those that can effectively enhance natamycin
production in shake-flask culture by Streptomyces natalensis upon addition to the medium. A mathematical model was built
using Box-Behnken experimental model and used to determine the optimal concentrations of selected plant hormones in the
medium. The results showed that indole-3-acetic acid, gibberellin and 6-benzyl amino purine effectively increased natamycin
production. At optimized concentrations of 5.5, 14.8 and 20.5 mg/L for these plant hormones, respectively, the yield of
natamycin was 1.805 g/L, which was 1.63 times higher than that of the control group.