鸡枞菌精多糖对酒精性损伤小鼠肾及免疫器官的抗氧化作用

目的：研究鸡枞菌精多糖（refined polysaccharide from Termitomyces albuminosus，RTAP）对酒精所致急性肝损伤小鼠肾、脾脏及胸腺的保护作用。方法：采用超声波辅助的热水浸提法提取鸡枞菌多糖，小鼠被随机分为正常组、模型组、阳性对照组（饲喂联苯双酯，150 mg/（kg·d））、RTAP各剂量组（100、200、400 mg/（kg·d）），连续灌胃30 d，正常组按等量生理盐水灌胃。第31天除了正常组外，给予50%乙醇（12 mL/kg）建立动物急性肝损伤模型。小鼠处死后取肾脏、脾脏、胸腺，测定各项抗氧化指标。结果：与模型组相比，RTAP各剂量组均能降低肾脏、脾脏及胸腺丙二醛含量，提高各器官超氧化物歧化酶活性、过氧化氢酶活性、谷胱甘肽过氧化物酶活性及谷胱甘肽含量。结论：RTAP对酒精性损伤小鼠肾及免疫器官具有明显的抗氧化作用，其原理应该与其所具有抗脂质过氧化和清除体内过多的氧自由基作用有关。

Antioxidant Effect of Polysaccharide from Termitomyces albuminosus on Kidney and Immune Organs in Mice with Alcoholic Hepatic Injury

Objective: The protective effect of a refined polysaccharide fraction from Termitomyces albuminosus (RTAP) on
kidney, spleen and thymus in mice with acute alcoholic hepatic injury was investigated. Methods: The refined polysaccharide
from Termitomyces albuminosus was extracted by ultrasound-assisted hot water. Mice were randomly divided into blank
control group, alcoholic injury model group, positive control group (administered with 150 mg/(kg·d) bifendate by gavage)
and RTAP groups at doses of 100, 200 and 400 mg/(kg·d). All mice were administered for 30 days prior to administration
of 50% alcohol 12 mL/(kg·d) except that the blank control group was given an identical volume of saline. After alcohol
treatment, all mice were sacrificed through cervical dislocation and subjected to analysis of antioxidant capabilities in the
kidney, spleen and thymus. Results: Compared with the model control group, the contents of MDA in kidney, spleen and
thymus were obviously decreased, while the contents of GSH and activities of SOD, CAT and GSH-Px were obviously
increased in the RTAP treatment groups. Conclusion: RTAP has antioxidant effect on kidney and immune organs in mice
with alcoholic hepatic injury, and the mechanisms might be associated with its antioxidant activity.