高效液相色谱法测定菠萝蜜果实中的葡萄糖、果糖和蔗糖

研究菠萝蜜果实葡萄糖、果糖和蔗糖的提取方法并对不同果实部位的糖进行分离，建立能同时测定菠萝蜜果实中3 种糖的高效液相色谱方法。CHO-820钙型糖柱为分析柱，柱温90 ℃，流动相为超纯水，流速0.5 mL/min，进样体积10 μL，用示差折光检测器检测。结果表明：100%乙醇是蔗糖的适宜提取液，若同时测定3 种糖，则最适提取液为80%乙醇溶液，方法的相对标准偏差为1.51%～7.01%，相关系数在0.999 0以上，加标回收率为98.55%～102.33%；菠萝蜜果肉（以鲜质计算）中3 种糖总含量高达14.01 g/100 g，其中蔗糖含量最高，种皮中糖总含量为10.19 g/100 g，以葡萄糖和果糖为主，果腱中含量最低，仅3.54 g/100 g。不同果实部位果糖与葡萄糖含量相近。

Determination of Glucose,Fructose and Sucrose in Jackfruit by HPLC

Sugars including glucose, fructose and sucrose in different fruit tissues of jackfruit were extracted by 3 different
extraction methods and determined by high performance liquid chromatography (HPLC). The HPLC conditions were set as
follows: the chromatograph was equipped with a CHO-820(Ca) column (300 mm × 7.8 mm) and a refractive index detector;
ultra-pure water was used as eluent with a flow rate of 0.5 mL/min; the column temperature was 90 ℃. Results showed
that 100% ethenol was the optimum extraction solvent for sucrose while 80% ethanol was the best solvent for simultaneous
extraction of glucose, fructose and sucrose. The relative standard deviations were in the range from 1.51% to 7.01%, and
the recoveries for spiked samples ranged from 98.55% to 102.33% and the correlation coefficients were higher than 0.999 0
for linearity, showing that the method is effective to isolate the three sugars from jackfuit. Results also showed that jackfruit
pulp contained 14.01 g/100 g mf sugars on a fresh weight basis with sucrose being the main component while the seed coat
contained 10.19 g/100 g mf, with fructose and glucose being the main components and Guojian (developed from unfertilized
or partly fertilized female perianth tissues) contained only 3.54 g/100 g mf. The fructose contents were similar to sucrose
contents in the 3 different tissues.