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| 响应面法优化苦荞皮中总黄酮超声波提取工艺 | |
| 引用本文： | 赵强,索有瑞,李天才,赵海福,董晓宁. 响应面法优化苦荞皮中总黄酮超声波提取工艺[J]. 食品科学, 2014, 35(16): 64-70. DOI: 10.7506/spkx1002-6630-201416012 |
| 作者姓名： | 赵强 索有瑞 李天才 赵海福 董晓宁 |
| 作者单位： | 1.中国科学院西北高原生物研究所，青海 西宁 810001；2.天水师范学院生物工程与技术学院，甘肃 天水 740001；3.甘肃农业大学动物医学院，甘肃 兰州 730070 |
| 基金项目： | 2013年甘肃省科技计划资助项目(1308RJZE305) |
| 摘 要： | 以苦荞皮为研究对象，在单因素试验基础上，采用Box-Behnken响应面试验设计法，对苦荞皮中总黄酮提取工艺条件进行研究，并对提取物进行高效液相色谱法检测。结果表明，影响苦荞皮中总黄酮含量的因素大小顺序为乙醇体积分数、液料比、回流时间、超声时间，最佳提取工艺为乙醇体积分数65%、液料比14∶1（mL/g）、回流时间1.6 h、超声时间28 min，苦荞皮中总黄酮最高含量为1.495 20%，高效液相色谱法检测重复性良好。 |
| 关 键 词： | 苦荞皮 总黄酮 响应面法 高效液相色谱法 |
Optimization by Response Surface Methodoloy of Ultrasonic Extraction of Total Flavonoids from Tartary Buckwheat Hull |
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| ZHAO Qiang,SUO You-rui,LI Tian-cai,ZHAO Hai-fu,DONG Xiao-ning. Optimization by Response Surface Methodoloy of Ultrasonic Extraction of Total Flavonoids from Tartary Buckwheat Hull[J]. Food Science, 2014, 35(16): 64-70. DOI: 10.7506/spkx1002-6630-201416012 | |
| Authors: | ZHAO Qiang SUO You-rui LI Tian-cai ZHAO Hai-fu DONG Xiao-ning |
| Affiliation: | 1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;2. College of Bioengineering and Technology, Tianshui Normal University, Tianshui 740001, China;3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China |
| Abstract: | The ultrasonic extraction of total flavonoids from tartary buckwheat hull was optimized by response surface analysis with Box-Behnken design, and the flavonoid extract obtained was analyzed by high performance liquid chromatography (HPLC). The results showed that the extraction efficiency of total flavonoids was affected in decreasing order by ethanol concentration, liquid-to-solid ratio, refluxing time and ultrasonication time, for which the optimal conditions were 65%, 14:1 (mL/g), 1.6 h and 28 min, respectively. Under the optimized conditions, the yield of total flavonoids was 1.495 20%. The HPLC method used in this study was found to be highly repeatable. |
| Keywords: | tartary buckwheat hull total flavonoids response surface methodology high performance liquid chromatography (HPLC) |
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