高速逆流色谱法分离绿茶中的化学成分

采用高速逆流色谱（high-speed countercurrent chromatography，HSCCC）法对绿茶化学成分进行分离。通 过对绿茶有效部位的分段萃取和多种溶剂系统的筛选，建立了绿茶化学成分HSCCC分离的方法。经HSCCC分离得 到咖啡碱、表没食子儿茶素、表儿茶素、儿茶素、表没食子儿茶素没食子酸酯、没食子儿茶素没食子酸酯、表儿茶 素没食子酸酯、没食子酸8 种单体化合物，其纯度分别为99.5%、97.2%、98.2%、97.6%、98.8%、99.1%、99.2%和 98.8%。该法分离效率高、操作简单，对绿茶在食品医药等领域的应用具有重要意义。

Application of High-Speed Countercurrent Chromatography in Purification of Chemical Constituents from Green Tea

Chemical constituents were separated from green tea by high-speed counter-current chromatography (HSCCC).
Based on stagewise extraction and optimization of solvent systems, the separation of active ingredients by HSCCC was
developed. Eight compounds including CFA, EGC, EC, C, EGCG, GCG, ECG and GA with high purity were obtained.
The purity of each compound was up to 99.5%, 97.2%, 98.2%, 97.6%, 98.8%, 99.1%, 99.2% and 98.8%, respectively. The
method is of high separation efficiency, simple operation, and great importance for the purification of components from
green tea in the food and medical fields.