Interfacial and biological properties of the gradient coating on polyamide substrate for bone substitute |
| |
Authors: | Di Huang Lulu Niu Yan Wei Meiqing Guo Yi Zuo Qin Zou Yinchun Hu Weiyi Chen Yubao Li |
| |
Affiliation: | 1.Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People''s Republic of China;2.Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People''s Republic of China |
| |
Abstract: | Fabrication of bioactive and mechanical matched bone substitutes is crucial for clinical application in bone defects repair. In this study, nano-hydroxyapatite/polyamide (nHA/PA) composite was coated on injection-moulded PA by a chemical corrosion and phase-inversion technique. The shear strength, gradient composition and pore structure of the bioactive coating were characterized. Osteoblast-like MG63 cells were cultured on pure PA and composite-coated PA samples. The cells'' adhesion, spread and proliferation were determined using MTT assay and microscopy. The results confirm that the samples with the nHA/PA composite coating have better cytocompatibility and have no negative effects on cells. To investigate the in vivo biocompatibility, both pure PA and composite-coated PA cylinders were implanted in the trochlea of rabbit femurs and studied histologically, and the bonding ability with bone were determined using push-out tests. The results show that composite-coated implants exhibit better biocompatibility and the shear strength of the composite-coated implants with host bone at 12 weeks can reach 3.49 ± 0.42 MPa, which is significantly higher than that of pure PA implants. These results indicate that composite-coated PA implants have excellent biocompatibility and bonding abilities with host bone and they have the potential to be applied in repair of bone defects. |
| |
Keywords: | interfacial properties gradient coating polyamide nano-hydroxyapatite osteogenesis |
|
|