桑黄胞外多糖药理活性的初步研究

目的与方法:MTT法测定桑黄胞外多糖(PIEP)对肿瘤细胞增殖反应的影响,研究其体外抗肿瘤活性;采用小鼠骨髓嗜多染红细胞微核实验和小鼠精子畸形实验,研究PIEP抗环磷酰胺(CP)致突变作用;通过测定创伤小鼠血清﹑肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,探讨其抗脂质过氧化作用。结果:PIEP能明显抑制肿瘤细胞的增殖,对肝癌细胞HepG-2、乳腺癌细胞MCF-7的半数抑制浓度(IC50)分别为194.42、261.56μg/ml;300、600mg/kgbw剂量的PIEP的微核抑制率可达44.78%和49.63%,各剂量组对CP诱发的精子畸形率也有显著抑制作用(p<0.01);PIEP还能明显增强创伤小鼠SOD活性,降低MDA含量。结论:PIEP有明显的抗肿瘤活性和抗脂质过氧化作用,对由环磷酰胺引起的体细胞和生殖细胞的基因突变有着明显的拮抗作用。

Preliminary Study on Pharmacological Activities of Exopolysaccharides of Phellinus igniarius

Objective and Methods: To study the anti-tumor effects of exopolysaccharides of Phellinus igniarius(PIEP), the effects of PIEP on the proliferation of tumour cell lines were assayed in vitro with MTT colorimetric method. Micronucleus tests in mouse bonemarrow polychromatic erythrocyte (PCE) and sperm abnormalities tests were used to researrch antimutaiton induced by cyclophosph--amide(CP). The activities of superoxide dismulase (SOD) and the content of malondialdehyde(MDA) in serum and liver of traumatic mice were respectively assayed to study their effects on lipid peroxide. Results: PIEP can inhibit proliferation of tumour cell significantly: IC50 to HepG-2 and MCF- 7 were 194.42; and 261.56μg/ml respectively; at doses of 300, 600mg/kg bw, MN inhibition rate can reach 44.78% and 49. 63% respectively; in comparison with CP group, PIEP can reduce sperm abnormality frequency significantly (p< 0.01) and PIEP can increase the activity of SOD and decrease the content of MDA in serum and liver of traumatic mice distinctly. Conclusion: PIEP has significant anti-tumour activity and lipid peroxide resisting effects. It can also inhibit gene mutation of body cells and germ cells induced by CP.