舒巴克坦对头孢哌酮体外抗生素后效应的影响

目的 考察合用β-内酰胺酶抑制剂舒巴克坦(SBT)前后头孢哌酮(CPZ)对4种致病菌抗生素后效应(PAE)的变化。方法 采用特异性 β一内酰胺酶鉴定试剂Nitrocefin挑选大肠埃希氏杆菌、金黄色葡萄球菌、变形杆菌和绿脓假单胞菌的产酶菌株,用比浊法测定受试菌株在不同CPZ浓度时的PAE值。结果 合用 SBT后,CPZ对产酶菌株的PAE均有不同程度的延长,低浓度时(1/2,1倍MIC)PAE增加更明显(P<0.05)。结论 合并CPZ和SBT的给药方案对治疗产β-内酰胺酶菌株引起的感染有利,尤其在体内药物浓度较低时,合并用药可以更长时间地抑制细菌生长。

In vitro postantibiotic effects induced by cefoperazone alone and in combination with sulbactam

Aim The postantibiotics effects (PAEs)induced by cefoperazone (CPZ)alone were to be compared with those induced by a 1:1 combination of CPZ and sulbactam(SBT).Methods Ail the clinical isolates selected, including every six isolates of E.coli, S.aureus, Proteus and P.aeruginosa, were proved to be beta-lactamase-producing strains by the Nitrocefin Test.The PAEs were determined by means of measurement of transmittance using AVANT AGE Microbiology System.Results Longer PAEs induced by the combination of CPZ and SBT were observed in each concentration, even in lower CPZ concentrations (1/2 and 1 MIC), the PAEs were significant longer than by CPZ alone.Conclusion The results suggest that the efficacy of CPZ combined with SBT is stronger than that of CPZ alone against the infections caused by beta-lactamase-producing isolates.