右旋普蔡洛尔对家兔和大鼠阴道内用药的耐受性及其对血糖、脂代谢和性激素水平的影响

目的 研究国产右旋普蔡洛尔(D-porparnolol,D-P)阴道内给药对家兔和大鼠的衬受性及其对血糖,脂代谢和性激素水平的影响。方法 家兔组: 12只家兔分4组,3只予以D-P栓剂(每个栓剂含D-P60mg和适量的基质聚乙二醇 1000)阴道内插入给药,每天一次,连续10天,并与阳性对照组(氛胺),阴性对照组(单纯机械刺激)和基质组(聚乙二醉)作比较;大鼠组: 12只大鼠给予阴道内注入D-P 0.2ml(40mg/ml) 每日一次,连续56天,每2周采血一次,测血糖、血脂和性激素浓度(E₂,P,LH,FSH)。实验结束后,立即处死动物,解剖阴道,用肉眼和显微镜检查阴道组织受损情况。结果 在家兔组未见阴道组织有异常损害,与正常对照组比较无明显差异(P>0.05),大鼠组可见阴道组织有轻微充血、水肿,但均在可耐受评分值内,对血糖血脂和性激素水平均无明显影响。结论 国产D-P阴道内给药对家兔和大鼠均无明显影响,说明阴道内给药是安全的。

Tolerance test of D-propranolol and its effect on sugar,lipid metabolism and sex hormone levels by intravaginal administration in rats and rabbits

Aim To study the tolerance of D-propranolol and is effect on sugar lipid metabolism and sex hormone levels by intravaginal administration in rats and rbbits.Methods 1.Rabbit group:three rabbits were given D-propranolol suppository (each suppository contain D-propranolol 60mg and opporture amount polyethanediol)by vaginal insertion, q.d. for 10 days. 2.Rat group:12 rats were given D-propranolol solution (40mg/ml)0.2 ml by direct injection into rat vagina q.d. for 56 days and blood samples were collected for measurement of blood sugar, lipids and sex hormone levels every 2 weeks.Immediately after killing all subject animals.Vaginal tissues were examined by macroscopy and microscopy.Results In the rabbits and rats group, no marked lesions of vaginal tissues were observed and the tissues did not significantly differ from those in the control group (P>0.05)and in rats group were slightly affected, i.e.mucosa edema and /or congestion but all those changes could be tolerated ,and there were no changes in blood sugar, lipids and sex hormones after treatmetn with D-propranolol for 8 weeks.Conclusions The results suggested that D-propranolol is safe when used in vagina.