维生素E对大鼠残肾纤维化的抑制作用

目的 观察维生素E对慢性肾衰残肾组织进行性纤维化的抑制作用。方法 在5/6肾切除大鼠慢性肾衰模型上, 以假手术大鼠为对照, 采用形态计量和生化测定方法, 对比分析术后30、60、90、120 天给予或不给予维生素E 治疗的慢性肾衰大鼠残肾纤维化程度。结果 大鼠行5/6 肾切除术后, 在残存肾小球代偿性肥大、肾小球毛细血管代偿性增生的同时, 肾小球显著硬化, 滤过膜显著增厚, 肾间质显著纤维化, 肾组织羟脯氨酸含量显著增高;而用维生素E 治疗的大鼠, 肾小球硬化、滤过膜增厚、肾间质纤维化和肾组织羟脯氨酸含量增高的程度显著减轻, 肾小球代偿性肥大和肾小球毛细血管代偿性增生的发生时间后移。结论 维生素E 能显著抑制5/6 肾切除所致慢性肾衰残肾组织的纤维化, 对其代偿性肾小球肥大和毛细血管增生无明显直接影响。

Inhibitory effect of vitamin E on fibrosis of remnant kidney

Aim To investigate whether vitamin E could suppress the fibrosis of remnant kidney in chronic renal failure (CRF)rats.Methods The 60 Wistar rats were divided into 3 groups. Rats in group A were experinced a sham operation serving as the negative control.rats in group B were given a 5/6 nephrectomy as the positive control.rats in group C were operated as those in group B and received treatment of vitamin E in their chow.The renal fibrosis of every group was estmated with the morphometry and biochemical methods 30, 60, 90, 120 days after operation respectively.Results In group B, accompanied with compensatory glome rular hypertrophy and glomerular capillary proliferation, there were obvious glomerular sclerosis, filtration membrane thickening, interstitial fibrosis, and renal hydroxy proline content increase.In group C, there were significantly less severe glomerular sclerosis, filtration membrane thickening, interstitial fibrosis, and renal hydroxy proline content than in group B.As a result, the glomerular hypertrophy and glome rular capillary proliferation were postponed.Conclusion Vitamin E can suppress fibrosis of remnant kidney in 5/6 nephrectomy induced CRF rats, and does not affect directly the compensatory glomerular hypertrophy and glome rular capillary proliferation.