| Vero细胞森林脑炎疫苗毒种的选育及其生物学特性检测 |
| |
| 引用本文： | 宋宗明,刘双军,王丽娜,赵洪丽,王伟,刘景云,洪成龙,惠连,李彤,韩亮.Vero细胞森林脑炎疫苗毒种的选育及其生物学特性检测[J].粉末涂料与涂装,2006,19(1):52-53. |
| |
| 作者姓名： | 宋宗明 刘双军 王丽娜 赵洪丽 王伟 刘景云 洪成龙 惠连 李彤 韩亮 |
| |
| 作者单位： | 长春生物制品研究所 长春130062 |
| |
| 摘 要： | 目的选育Vero细胞森林脑炎疫苗适应毒种。方法将森林脑炎疫苗鼠脑病毒“森张”株在Vero细胞上连续传代适应,并对不同代次收获的病毒液的病毒滴度、免疫原性、稳定性等进行检测。结果“森张”株病毒在Vero细胞上传代适应后,病毒滴度达8·5LgLD50/ml;免疫血清中和抗体效价高于1∶20,其它检测项目均符合疫苗生产用毒种的要求。结论Vero细胞适应的“森张”株毒种的初步特性显示可作为制备Vero细胞森林脑炎疫苗用毒种。
|
| 关 键 词： | 毒种选育 Vero细胞 免疫原性 生物学特性 |
| 收稿时间： | 2005-03-09 |
| 修稿时间： | 2005年3月9日 |
Screening and Biological Characteristics of Virus Seed for Preparation of Vero cell-derived Tick-borne Encephalitis Vaccine |
| |
| SONG Zong-ming, LIU Shuang-jun, WANG Li-na, et al.Screening and Biological Characteristics of Virus Seed for Preparation of Vero cell-derived Tick-borne Encephalitis Vaccine[J].Chinese Journal of Biologicals,2006,19(1):52-53. |
| |
| Authors: | SONG Zong-ming LIU Shuang-jun WANG Li-na |
| |
| Affiliation: | Changchun Institute of Biological Products, Changchun 130062, China |
| |
| Abstract: | Objective To screen an appropriate virus seed for the preparation of Vero cell-derived tick-borne encephalitis vaccine.Methods The current virus strain Sen-Zhang for preparation of tick-borne encephalitis vaccine was subcultured in Vero cells,and the titers,immunogenicities and stabilities of virus of various passages were studied.Results The virus titer of Sen-Zhang strain adapted in Vero cells reached 8.5 LgLD_ 50 /ml.The serum neutralizing antibody titer of mice immunized with the strain reached more than 1∶20 .All the quality indexes of the adapted strain met the requirements for virus seeds for vaccine production.Conclusion The Vero cell-adapted Sen-Zhang stain might be used as a virus seed for preparation of Vero cell-derived tick-borne encephalitis vaccine. |
| |
| Keywords: | Virus seed Vero cell Immunogenicity Biological characteristics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
